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ｐ５３基因是一种抑癌基因，ＭＤＭ２基因的产物是ｐ５３的结合蛋白，可使ｐ５３抑癌基因失活。了解骨肉瘤ｐ５３、ＭＤＭ２基因转录水平上的变化，可促进对肿瘤发生的基因水平变化的了解。作者用生物素标记的ｐ５３寡核苷酸探针及ＭＤＭ２寡核苷酸探针，将２０例骨肉瘤的ｐ５３、ＭＤＭ２肿瘤组织制成细胞悬液，经甩片机制成细胞甩片，４％多聚甲醛固定、蛋白酶Ｋ破坏细胞膜，常规方法进行原位杂交，金标链亲和素染色系统染色，核固红复染。２０例骨肉瘤中，８例有ｐ５３基因的高表达，占４０％；６例有ＭＤＭ２基因的高表达，占３０％；其中２例ｐ５３、ＭＤＭ２基因表达均增高。以上结果表明骨肉瘤存在有ｐ５３、ＭＤＭ２基因表达的异常。
The p53 gene is a tumor suppressor gene. The product of the MDM2 gene is a p53 binding protein that inactivates the p53 tumor suppressor gene. Understanding the changes in the transcriptional levels of p53 and MDM2 genes in osteosarcoma can promote the understanding of the changes in gene levels of tumorigenesis. The authors used biotin-labeled p53 oligonucleotide probes and MDM2 oligonucleotide probes to make 20 tumors of p53 and MDM2 tumours into cell suspensions, which were then made into a cell suspension by a spin-casting machine. 4% Paraformaldehyde was fixed and proteinase K was used to destroy the cell membrane. In situ hybridization was performed by conventional methods, gold streptavidin staining system was used, and nuclear solid red stained. In 20 cases of osteosarcoma, 8 cases had high expression of p53 gene, accounting for 40%; 6 cases had high expression of MDM2 gene, accounting for 30%; 2 cases of p53, MDM2 gene expression were increased. The above results indicate that there are abnormal expressions of p53 and MDM2 genes in osteosarcoma.