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The incidence of iatrogenic splenic injury during gynecological laparoscopic surgery is rare or probably underreported. The spleen is situated principally in the left hypochondrium, connected to the colon, the omentum majus and the lateral abdominal wall. The estimated prevalence of iatrogenic trauma after the more common operative procedures is considered relatively low, however, immediate identification and management either by splenic repair or splenectomy should result in avoiding any potential complications.We presented a case of uterine myoma enucleation of the anterior wall of the uterus with an accidental injury of the spleen that implicated splenectomy. Constant awareness of the prevalence of this operative complication and the mechanisms by which it may arise should enable its early identification and prevention of any potential sequelae.