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研究目的 探讨幼年型类风湿性关节炎与血清IgG亚类改变的关系。 研究设计 病例对照研究。 患者和其它参与者 幼年型类风湿性关节炎患儿共16例,男8例,女8例。年龄5～13岁。对照组30例,男15例,女15例。年龄5～13岁,均为健康儿童。 处理方法 用多克隆抗体单向免疫扩散法测定血清IgG、IgM、IgA浓度;用双向酶联免疫法测定血清中IgG亚类。 结果 5～9岁对照组和观察组血清IgG分别为9.90±2.15和14.10±6.169/L(t=3.832,P<0.01),IgA为1.04±0.33和0.99±0.61g/L(t=0.357,P>0.5),IgM为1.47±0.36和1.50±0.65g/L(t=0.200,P>0.5)。10～13岁对照组和观察组IgG、IgA、IgM与此相近。5～9岁对照组和观察组IgG_1分别为5.501±0.976和9.715±3.746g/L(t=5.046,P<0.01),IgG_2分别为2.664±0.765和2.715±0.909g/L(t=0.146,P>0.05),lgG_3分别为0.517±0.167和0.828±0.578g/L(t=2.132,P<0.05),IgG_4分别为0.300±0.093和0.596±0.632 g/L(t=2.074,P>0.05)。10～13岁观察组IgG_2显著高于对照组(P<0.01)。 结论 类风湿性关节炎患儿体内IgG水平显著增高,IgG亚类也明显增高,活动期患儿IgG_1IgG_3和IgG_4增加明显。
Objective To investigate the relationship between juvenile rheumatoid arthritis and changes of serum IgG subclasses. Study design Case-control study. There were 16 patients with juvenile rheumatoid arthritis in patients and other participants, 8 males and 8 females. Age 5 to 13 years old. Control group, 30 cases, 15 males and 15 females. Age 5 to 13 years old, are healthy children. Methods The serum IgG, IgM and IgA concentrations were measured by one-way immunodiffusion with polyclonal antibody. The IgG subclasses were determined by two-dimensional enzyme-linked immunosorbent assay. Results Serum IgG in control and observation groups aged 5 to 9 years were respectively 9.90 ± 2.15 and 14.10 ± 6.169 / L (t = 3.832, P <0.01), IgA was 1.04 ± 0.33 and 0.99 ± 0.61g / L P> 0.5), IgM was 1.47 ± 0.36 and 1.50 ± 0.65 g / L (t = 0.200, P> 0.5). 10 ~ 13-year-old control group and observation group IgG, IgA, IgM similar. IgG1 in control group and observation group were 5.501 ± 0.976 and 9.715 ± 3.746g / L (t = 5.046, P <0.01), IgG2 was 2.664 ± 0.765 and 2.715 ± 0.909g / (T = 2.132, P <0.05), and IgG4 were 0.300 ± 0.093 and 0.596 ± 0.632 g / L (t = 2.074, P> 0.05) . IgG 2 in observation group 10 to 13 years old was significantly higher than that in control group (P <0.01). Conclusion The level of IgG in children with rheumatoid arthritis is significantly increased, IgG subclasses are also significantly increased, and IgG1 IgG3 and IgG4 are significantly increased in children with active rheumatoid arthritis.