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Cryopreservation of Porphyra yezoensis conchocelis was conducted with cryoprotectants and a proposed pretreatment procedure and thawing methods explored. Six cryoprotectants combined by DMSO with ethylene glycol(EG),propylene glycol(PEG),sorbitol and sucrose were developed. The effect of prefreezing at - 40℃ or -20℃ for different time durations was compared and the thawing methods were screened. It was shown that the cryoprotectant including 10% DMSO with 0.5 molL-1 sorbitol exhib-ited the optimal effect. The ideal pretreatment was that conchocelis segments were stayed for 20 min at - 40℃ before stored in liquid nitrogen,and 40℃ water bath was proper for quick thawing. The highest recovery rate of cryopreserved P. yezoensis conchocelis reached 89.41%.
Cryopreservation of Porphyra yezoensis conchocelis was conducted with cryoprotectants and a proposed pretreatment procedure and thawing methods explored. Six cryoprotectants combined by DMSO with ethylene glycol (EG), propylene glycol (PEG), sorbitol and sucrose were developed. The effect of prefreezing at - 40 ℃ or -20 ℃ for different time durations was compared and the thawing methods were screened. It was shown that the cryoprotectant containing 10% DMSO with 0.5 mol L -1 sorbitol exhib- ited the optimal effect. The ideal pretreatment was that conchocelis segments were for 20 min at - 40 ° C before stored in liquid nitrogen, and 40 ° C water bath was proper for quick thawing. The highest recovery rate of cryopreserved P. yezoensis conchocelis reached 89.41%.