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报道了一种新的肽类树枝状分子改性磁性纳米药物载体.以天然氨基酸L-谷氨酸为原料,通过收敛法合成了聚(L-谷氨酸)树状分子,将多巴胺配体键合到聚(L-谷氨酸)树状分子上,用核磁(1H-NMR)、质谱(MS)对合成出的树状分子配体进行了表征,然后通过配体交换对四氧化三铁磁纳米粒表面进行多功能化.以阿霉素为模型药物通过pH敏感的腙键与聚(L-谷氨酸)树状分子外围的官能团结合,将药物负载到磁纳米粒表面构建载药磁纳米粒.用透射电子显微镜(TEM)、动态光散射(DLS)、傅立叶红外光谱(FTIR)、选区电子衍射(SEAD)对纳米药物载体的微观形貌和结构进行了表征,研究结果显示表面功能化后的磁纳米粒在水相中具有很好的分散性和稳定性.纳米载药粒的平均粒径为63 nm,载药量约为52.4 mg g-1.药物释放研究发现,载药粒具有良好的pH响应性,在pH=7.4时释放较慢,而在pH=5.0的微酸性环境释放较快.体外细胞实验结果表明,树状分子改性后的磁纳米粒细胞毒性较低,而载药纳米粒对HeLa细胞的增殖有明显抑制作用.这种pH敏感的聚(L-谷氨酸)树状分子改性磁纳米粒可作为载体用于药物传输系统.
A novel peptide dendrimer-modified magnetic nanoparticle drug carrier was reported.A poly (L-glutamic acid) dendrimer was synthesized by the convergence method using L-glutamic acid, a natural amino acid, as a starting material, and dopamine ligand (L-glutamic acid) dendrimer. The synthesized dendrimer ligands were characterized by 1H-NMR and MS. Ferric nanoparticles on the surface of multi-functional drugs.Adriamycin as a model drug through the pH-sensitive hydrazone bond with the poly (L-glutamic acid) dendrimer peripheral functional groups, the drug loaded onto the surface of the magnetic nanoparticles loaded The magnetic nanoparticles were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR) and selected area electron diffraction (SEAD) The surface-functionalized magnetic nanoparticles have good dispersibility and stability in the aqueous phase.The average diameter of the nanoparticles loaded nanoparticles was 63 nm, the drug loading was about 52.4 mg g-1. Drug release study found that, Drug-loaded tablets have good pH-responsiveness, release slower at pH = 7.4, and slightly acidic rings at pH = 5.0 Release faster.Experimental results in vitro showed that dendrimer-modified magnetic nanoparticles have lower cytotoxicity and drug-loaded nanoparticles have a significant inhibitory effect on the proliferation of HeLa cells.This pH-sensitive poly (L- Amino) dendrimer-modified magnetic nanoparticles can be used as a carrier for drug delivery systems.