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目的:通过观察心乐胶囊对病毒性心肌炎小鼠基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶抑制剂-1(TIMP-1)蛋白水平的影响。方法:取60只BALB/c雄性小鼠随机分成六组:空白对照组、模型对照组、玉丹荣心丸组、心乐胶囊低、中、高剂量组。除空白组外,后5组造模。后药物组用药物干预15天,将小鼠处死后迅速留取心脏标本,采用免疫组化SP二步法检测小鼠心肌MMP-1、TIMP-1的表达。结果:心乐胶囊各剂量组及阳性药对照组均能降低柯萨奇病毒致病毒性心肌炎小鼠心肌组织MMP-1含量、同时增高心肌组织TIMP-1含量。心乐胶囊各剂量组与模型组相比有显著差异P<0.01,其中以心乐胶囊高剂量组最为明显。结论:心乐胶囊具有抑制MMP-1,提高TIMP-1的活性的作用,可以减轻心肌炎小鼠的心肌损害,有效防止心肌纤维化。
Objective: To observe the effect of Xinle Capsule on the levels of matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-1 (TIMP-1) in mice with viral myocarditis. Methods: Sixty BALB / c male mice were randomly divided into six groups: control group, model control group, YUDAN Rongxin Pill group and Xinle capsule low, medium and high dose groups. In addition to the blank group, the latter five groups modeling. After drug treatment for 15 days in the drug group, the heart samples were collected rapidly after the mice were sacrificed. The expression of MMP-1 and TIMP-1 in myocardium was detected by immunohistochemical SP method. Results: Each dose of Xinle Capsule and the positive control group could reduce the content of MMP-1 in myocardial tissue and increase the content of TIMP-1 in myocardial tissue of mice with viral myocarditis caused by coxsackievirus. Xinle Capsule dose group and the model group compared to a significant difference P <0.01, of which the high-dose Xinle capsule most obvious. Conclusion: Xinle capsule has the effect of inhibiting MMP-1 and increasing the activity of TIMP-1, and can reduce myocardial damage in myocarditis mice and effectively prevent myocardial fibrosis.