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目的 :研究p2 1WAF1、p16INK4a及CDK4在以乙型肝炎病毒 (HBV)感染为主要诱因的肝细胞癌 (HCC)中的改变及变化机理。方法 :利用免疫组织化学技术检测HCC中p2 1WAF1、p16INK4a及CDK4的蛋白表达 ;对HCC的实体瘤及血液进行HBV -DNA的PCR。结果 :( 1)p16INK4a蛋白在HCC中表达的阳性率为 5 1 3 5 % ,p2 1WAF1蛋白的表达率为 4 3 2 4 % ,而正常肝组织中为 10 0 % ,两组均有显著差异 (P <0 0 5 )。p16INK4a蛋白在伴转移HCC的表达率为2 3 0 8% ,显著低于未转移的HCC中p16INK4a的蛋白阳性率 ( 71 4 3 % ) (P <0 0 5 ) ;( 2 )p16INK4a蛋白在HBV阳性的HCC中的表达阳性率为 5 3 5 7% ,在HBV阴性的HCC中的表达率为 5 0 %。p2 1WAF1在这两组中的表达率分别为 5 0 %及3 3 3 3 % (P >0 0 5 )。结论 :p16INK4a及p2 1WAF1蛋白的缺失与HCC的发生发展有关 ,与HBV的感染没有相关性。p16INK4a蛋白的缺失与HCC转移有关
Objective: To investigate the changes and mechanisms of p2 1WAF1, p16INK4a and CDK4 in hepatocellular carcinoma (HCC) with hepatitis B virus (HBV) infection as the main cause. Methods: The expression of p21WAF1, p16INK4a and CDK4 in HCC was detected by immunohistochemical technique, and the HBV-DNA PCR was performed on solid tumors and blood of HCC. Results: (1) The positive rate of p16INK4a protein in HCC was 5 1 3 5 %, the expression rate of p2 1WAF1 protein was 4 3 2 4 %, while in normal liver tissue it was 100 %. There were significant differences between the two groups. (P < 0 0 5). The expression rate of p16INK4a protein in HCC with metastasis was 238.8%, which was significantly lower than that of p16INK4a protein in non-metastatic HCC (71 4 3%) (P < 0.05). (2) p16INK4a protein in HBV The positive expression rate in positive HCC was 5337%, and the expression rate in HBV-negative HCC was 50%. The expression rate of p2 1WAF1 in these two groups was 50% and 3 3 3 3% (P > 0 05). Conclusion : The deletion of p16INK4a and p2 1WAF1 protein is related to the occurrence and development of HCC and has no correlation with HBV infection. Loss of p16INK4a protein is associated with HCC metastasis