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材料与方法 一、材料 试验于1994年1月1日在华南农业大学生物物理实验室(1)实验地进行,盆裁两年生‘糯米糍’荔枝(淮枝砧)3株,1月6日分别进行主干螺旋环剥(剥树皮宽2mm,1.2圈,螺距10cm,深达木质部),环剥(宽2mm,闭口一圈),并设对照(对主干不做任何处理)。处理前并用塑料覆盖加温。 二、方法 1、喂饲:于1994年1月6日下午每株树冠顶部强健的枝一条,有75片小叶,将该枝装入一透明密封塑料小室中,然后向小室反应瓶中加人75μciNa_2~(14)CO_3溶液和足量的1N盐酸,让其反应完全,4小时后中止喂饲,用5NNaOH回收过量的~(14)CO_2。 2、取样:(1)喂饲部位:分别在喂饲后0小时、48小时和72小时三个时间取源叶(小叶)25片及相应的枝;(2)非喂饲部位:分别在
MATERIALS AND METHODS I. Material testing was conducted experimentally on January 1, 1994 in the Laboratory of Biophysics, South China Agricultural University (1). Three biennial ’Glutinous Rice’ litchi Spiral barking (stripping bark 2mm wide, 1.2 laps, pitch 10cm, deep xylem), girdling (width 2mm, closed lap), and the control (no treatment of the trunk). Before treatment and use plastic cover heating. Method 1. Feeding: On the afternoon of January 6, 1994, a strong branch on the top of each canopy, with 75 leaflets, was placed in a clear, sealed plastic cell and then added to a small reaction flask 75μciNa_2 ~ (14) CO_3 solution and a sufficient amount of 1N hydrochloric acid were added to complete the reaction. After 4 hours, feeding was stopped and the excess of ~ (14) CO_2 was recovered with 5N NaOH. (2) Sampling: (1) Feeding site: Take 25 pieces of leaves (leaflets) and corresponding branches at 0, 48 and 72 hours after feeding respectively; (2) Non-feeding sites: