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Objective:To investigate the preliminary phytochemistry and antibacterial activity of the flower extract ofPeltophorum pterocarpum.Methods: Phytochemical analysis was done by using the standard methods given by Harbone. The methanolic flower extract were tested against Escherichia coli,Klebsiella pneumonia, Pseudomonas aeruginosa,Staphylococcus aureus, Bacillus cereus,Salmonella typhi,Serratia marsecens,Acinetobacter baumannii,Enterobacter sp., Proteus mirabilis,Enterococcus faecalis andStreptococcus pyogenes by the agar disc diffusion method.Results: Preliminary phytochemical screening of flower extract showed the presence of phenolic compounds, flavonoids, saponins, steroids, tannins, xanthoproteins, carboxylic acids, coumarins and carbohydrates. The flower extract of Peltophorum pterocarpum showed significant activity against four gram positive (Staphylococcus aureus,Bacillus cereus,Enterococcus faecalis andStreptococcus pyogenes) and three gram negative bacteria (Proteus mirabilis, Acinetobacter baumannii and Serratia marsecens), out of 12 pathogenic bacteria studied.Conclusions: The findings of the present study confirm the presence of significant antibacterial activity against human pathogens in the flowers ofPeltophorum pterocarpum.