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目的 :测定汉防己甲素在家兔血浆中的浓度。方法 :采用兔耳缘静脉注射给药方式 ,按不同时间间隔颈动脉取血 ,采用HypersilODS C18柱 ,以甲醇 水 ( 4 5∶5 5 )为流动相 ,检测波长 2 10nm ,以延胡索乙素为内标。结果 :标准曲线回归方程为Y =0 .5 318X + 0 .0 6 88,(γ =0 .996 2 ) ,汉防己甲素在 0 .5 6 2 5～ 18μg·ml-1浓度范围内与峰面积比值呈良好的线性关系 ,平均加样回收率 88.5 % ,日内及日间RSD分别为 3.1%和 4 .7%。结论 :本方法简便、快速、可靠 ,可为汉防己甲素的其他生物样品分析提供参考
Objective : To determine the concentration of tetrandrine in rabbit plasma. METHODS: Rabbit ear vein injection was used. Blood was taken from the carotid artery at different time intervals. Hypersil ODS C18 column was used with methanol water (45: 5 5) as the mobile phase. The detection wavelength was 210 nm. Tetrahydropalmatine was used as the mobile phase. Internal standard. Results: The regression equation of the standard curve was Y =0. 5 318X + 0.66, (γ =0.996 2 ), and tetrandrine was within the concentration range of 0.562 5-18 μg·ml-1. The peak area ratio showed a good linear relationship, the average recovery rate was 88.5 %, and the intraday and inter-day RSD were 3.1% and 4.7%, respectively. Conclusion : This method is simple, rapid, reliable and can provide reference for the analysis of other biological samples of tetrandrine.