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丙型肝炎病毒(HCV)是导致慢性非甲非乙型肝炎的主要病因,其核苷酸序列已经弄清,可编码3010个氨基酸多聚蛋白体,其基因产物的顺序为NH_2-p22-gp35-gp70-COOH,且p22和gp35分别是HCV的核心蛋白和包膜糖蛋白。作者用λgt11 eDNA克隆系统,在慢性非甲非乙型肝炎患者血清中得到含gp35基因的cDNA克隆,进而亚克隆入痘苗病毒转移载体,再将痘苗病毒和这种重组喷粒先后感染和转染兔肾(RK13)细胞,最后筛选出含
Hepatitis C virus (HCV) is the leading cause of chronic non-A, non-B hepatitis, the nucleotide sequence has been identified, can encode 3010 amino acid polyprotein body, the gene product of the order of NH_2-p22-gp35 -gp70-COOH, and p22 and gp35 are the core and envelope glycoproteins of HCV, respectively. Using the λgt11 eDNA cloning system, a cDNA clone containing the gp35 gene was obtained from the sera of patients with chronic non-A, non-B hepatitis and then subcloned into a vaccinia virus transfer vector. The vaccinia virus and the recombinant pneumoniae were successively infected and transfected Rabbit kidney (RK13) cells, and finally screened