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目的:研究阿霉素诱导的扩张型心肌病大鼠心律失常与内质网应激的关系。方法:30只Wister雄性大鼠分为对照组、阿霉素模型组(模型组),每组15只,对照组腹腔注射0.9%Nacl(10ml/kg),共3次(每周1次);模型组腹腔注射阿霉素2mg/kg,共注射3次(每周1次)。7周末2组大鼠行心电图检测心律失常发生率及心脏彩超检测左室舒张末径及左心室射血分数,2组大鼠处死后取左心室组织行苏木精-伊红、masson、VG染色,观察心肌病理改变,采用real time PCR及Western blotting技术检测2组大鼠心肌细胞内质网应激伴侣蛋白GRP78,GRP94表达。结果:与对照组大鼠比较,模型组大鼠心律失常发生率增多(P<0.05),心肌细胞内质网应激伴侣蛋白GRP78,GRP94表达明显增多(P<0.01)。结论:阿霉素诱导扩张型心肌病大鼠心律失常发生可能与心肌细胞内质网应激相关。
Objective: To investigate the relationship between adriamycin-induced dilated cardiomyopathy and arrhythmia in endoplasmic reticulum stress. Methods: Thirty Wister male rats were divided into control group and Doxorubicin model group (model group), 15 rats in each group. The control group received intraperitoneal injection of 0.9% Nacl (10ml / kg) for 3 times (once a week) ; Model group, intraperitoneal injection of doxorubicin 2mg / kg, a total of 3 times a week (once a week). The incidence of arrhythmia was detected by electrocardiogram (ECG) and the left ventricular end-diastolic diameter (LVEF) and left ventricular ejection fraction (LVEF) were measured in the two groups at 7weeks. The pathological changes of myocardium were observed. Real-time PCR and Western blotting were used to detect the expression of GRP78 and GRP94. Results: Compared with the control group, the incidence of arrhythmia in model group increased (P <0.05), and the expressions of GRP78 and GRP94 increased significantly in cardiomyocytes (P <0.01). Conclusion: Adriamycin-induced dilated cardiomyopathy rats arrhythmia may be related to endoplasmic reticulum stress.