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以150个不同生态类型的大豆品种(系)为试材,分别用4种腐霉菌进行接种鉴定,筛选抗病性,并用256个SSR(simple sequence repeat)标记对不同抗性类型大豆品种进行基因组扫描,分析群体结构和连锁不平衡,同时用TASSEL软件中的GLM方法对大豆4种腐霉菌的抗性进行关联分析,T测验检测差异显著位点,进而发掘各关联位点的优异等位基因。结果表明:①参试大豆品种与256个SSR位点存在广泛的连锁不平衡,而且共线位点D’衰退较快,适合于连锁不平衡分析。SSR数据结构和亲缘分析表明参试品种分为7个亚群。②用关联分析方法得到与4种腐霉菌抗性关联的引物25个,T测验和抗性验证找到差异显著的关联等位变异位点12个,它们在参试品种中都有较大的效应值。③关联分析鉴定差异显著的等位变异位点与实际品种抗病性结果高度吻合。其关联分析中可信度高的等位变异位点和T测验中显著的等位变异位点相吻合。即与P.aphanidermatum抗性关联的位点为Satt191-1、Satt584-1和Satt584-2;与P.irregulare抗性关联的位点为Satt602-1和Satt042-4。
150 soybean cultivars with different ecotypes were used as materials to inoculate the four kinds of Pythium to identify the disease resistance and 256 SSR (simple sequence repeat) markers were used to genotype different soybean varieties Scanning and analyzing population structure and linkage disequilibrium, we also analyzed the resistance of four kinds of Pythium in Pythium by TASSEL software. The T test detected significant differences in loci, and then discovered the excellent alleles . The results showed that: ① There was a wide range of linkage disequilibrium between the tested soybean varieties and 256 SSR loci, and the collinear line D ’had a fast decline, which was suitable for linkage disequilibrium analysis. SSR data structure and genetic analysis showed that the tested varieties were divided into seven subgroups. (2) Twenty-five primers were used to analyze the resistance of four Pythium strains by correlation analysis. Twelve significant allelic variants were found in the T test and the resistance test, which all had significant effects on the tested varieties value. ③ Correlation analysis identified significant differences in allelic variation sites and the actual varieties of disease resistance results are highly consistent. The correlation analysis of high confidence alleles and T-test significant allelic variations consistent. That is, the sites associated with P.aphanidermatum resistance were Satt191-1, Satt584-1 and Satt584-2; the sites associated with P. irregulare resistance were Satt602-1 and Satt042-4.