论文部分内容阅读
采用游离肝细胞培养技术,用非极性毛细管气相色谱法测定大白鼠肝细胞中CCl_4并分离其氟代甲烷类代谢物。方法线性范围宽(0.1mmol/L～0.01μmol/L),灵敏度高(最低检测浓度为5×10~(-3)nmol/L)。结果还提示,CCl_4在进入肝细胞后,被分解成CHCl_3、CH_2Cl_2等代谢产物;CHCl_3、CH_2Cl_2可能为CCl_4致肝损伤的除脂质过氧化外的又一条途径。
Free hepatic cell culture was used to determine CCl_4 in rat hepatocytes by non-polar capillary gas chromatography and to separate the fluoromethanes metabolites. The method has wide linear range (0.1mmol / L ~ 0.01μmol / L) and high sensitivity (the lowest detection concentration is 5 × 10 ~ (-3) nmol / L). The results also suggest that, after entering the liver cells, CCl_4 is decomposed into metabolites such as CHCl_3 and CH_2Cl_2. CHCl_3 and CH_2Cl_2 may be another pathway besides lipid peroxidation induced by CCl_4.