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目的:研究槲皮素(quercetin)是否对过氧化氢所致PC-12细胞氧化损伤具有保护作用,以及可能的保护机制。方法:用PC-12细胞建立H2O2氧化损伤模型;测定SOD、T-AOC生化指标判断细胞抗氧化能力;半定量RT-PCR法检测糖皮质激素受体(GR)基因转录水平。结果:①MTT结果:H2O2能使细胞活力显著降低(P<0.01),槲皮素的预孵处理能够明显减轻H2O2对PC-12细胞的氧化损伤(P<0.05&0.01)。②SOD活性结果:H2O2作为氧化损伤因素使得细胞SOD活性应激上升(P<0.01),Qu组细胞SOD活性维持在较低水平(P<0.01)。③T-AOC结果:槲皮素能够显著提高PC-12细胞的总抗氧化能力(P<0.01)。④半定量RT-PCR结果:H2O2使PC-12细胞GR基因转录水平显著降低,槲皮素能够减轻其转录所受影响(P<0.01)。结论:槲皮素与PC-12细胞的共孵育,提高了细胞整体的抗氧化能力,维持了GR基因的转录水平,继而保护细胞免受后续H2O2的氧化损伤和炎症反应,维持细胞生化环境的稳态。
AIM: To investigate whether quercetin has a protective effect on the oxidative damage of PC-12 cells induced by hydrogen peroxide and the possible protective mechanism. Methods: The oxidative damage model of H2O2 was established by PC-12 cells. The antioxidant capacity of SOD and T-AOC was determined by measuring the biochemical indexes of SOD and T-AOC. The transcription level of glucocorticoid receptor (GR) gene was detected by semi-quantitative RT-PCR. Results: ① MTT results: H2O2 significantly decreased cell viability (P <0.01). Pretreatment with quercetin significantly reduced H2O2-induced oxidative damage in PC-12 cells (P <0.05 & 0.01). (2) SOD activity results: H2O2 as a factor of oxidative damage increased the activity of SOD in cells (P <0.01), while the activity of SOD in Qu group maintained at a lower level (P <0.01). ③ T-AOC results: Quercetin can significantly increase the total antioxidant capacity of PC-12 cells (P <0.01). ④ Semi-quantitative RT-PCR results: H2O2 significantly reduced GR gene transcription in PC-12 cells, and quercetin could reduce the transcriptional effect (P <0.01). CONCLUSION: Co-incubation of quercetin with PC-12 cells increases the overall antioxidant capacity of cells and maintains the transcriptional level of GR gene, which in turn protects cells from subsequent oxidative damage and inflammatory responses of H2O2 and maintains the biochemical Steady state.