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目的:探讨银杏内酯B(GB)对缺氧缺血性脑损伤(HIBD)新生大鼠脑组织Foxg1 mRNA的表达及内源性神经干细胞增殖、分化的影响。方法:清洁级新生7天SD大鼠随机分为假手术组、模型组及GB干预组,后两组采用Rice法建立HIBD模型,造模后3、7、14和28天处死,实时荧光定量PCR(RT-PCR)法检测脑组织Foxg1 mRNA的表达,免疫组化染色法检测海马齿状回BrdU+细胞的表达,免疫荧光双标法检测大脑皮层BrdU+/Nestin+、BrdU+/GFAP+和BrdU+/NSE+细胞的表达。结果:HIBD后各时间点脑组织Foxg1 mRNA、BrdU+细胞、BrdU+/Nestin+细胞的表达GB干预组>模型组>假手术组,差异有统计学意义(P<0.01);各时间点脑组织BrdU+/NSE+细胞的表达GB干预组>模型组>假手术组,差异有统计学意义(P<0.01);各时间点脑组织BrdU+/GFAP+细胞的表达GB干预组>模型组>假手术组,差异有统计学意义(P<0.01)。结论:GB通过上调HIBD后脑组织Foxg1 mRNA的表达,促进神经干细胞增殖及分化,减轻并修复脑损伤。
Objective: To investigate the effect of ginkgolide B (GB) on the expression of Foxg1 mRNA and the proliferation and differentiation of endogenous neural stem cells in neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods: Seven-day-old SD rats were randomly divided into sham operation group, model group and GB intervention group. The latter two groups were established HIBD model by Rice method. The rats were sacrificed on days 3, 7, 14 and 28 after modeling. Real- The expression of Foxg1 mRNA in brain tissue was detected by RT-PCR. The expression of BrdU + cells in dentate gyrus of hippocampus was detected by immunohistochemical staining. BrdU + / Nestin +, BrdU + / GFAP + and BrdU + / NSE + cells were detected by double- expression. Results: The expression of Foxg1 mRNA, BrdU + cells and BrdU + / Nestin + cells at each time point after HIBD were significantly different in the intervention group, the model group and the sham operation group (P <0.01) (P <0.01). The expression of BrdU + / GFAP + cells in GB at each time point was significantly higher than that in GB intervention group> model group> sham operation group Statistical significance (P <0.01). CONCLUSION: GB can promote the proliferation and differentiation of neural stem cells and relieve and repair brain injury by up-regulating the expression of Foxg1 mRNA in brain tissue after HIBD.