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利用左氧氟沙星诱变铜绿假单胞菌NY3获得NY3-1和NY3-2诱变株,通过比较所产表面活性剂的理化性能可知,诱变株和野生株所产鼠李糖脂表面活性剂的产量、成分和结构特征有明显的差异。与野生菌株相比,NY3-1产量提高19.57%,NY3-2产量降低17.60%;NY3、NY3-1、NY3-2所产鼠李糖脂的双/单糖含量比例(即为斑点密度比)分别为0.969、0.677、1.316,红外光谱图显示,NY3所产鼠李糖脂中不含CC键,而经左氧氟沙星诱导后的NY3-1和NY3-2分泌的鼠李糖脂中均含有CC不饱和键。比较ESI-MS质谱发现,3种菌株所产鼠李糖脂中都含有Rh-C10,诱变菌株所产鼠李糖脂中含有不饱和键的Rha-Rha-C10:1,且含量较高。
The mutagenesis of NY3-1 and NY3-2 was carried out by levofloxacin mutagenesis of NY3-1 and NY3-2 mutants. By comparing the physical and chemical properties of the produced surfactants, the mutagenic and wild-type rhamnolipid surfactants There are obvious differences in yield, composition and structural characteristics. Compared with the wild strain, the yield of NY3-1 increased by 19.57% and the yield of NY3-2 decreased by 17.60%. The ratio of double / monosaccharide content of rhamnolipid produced by NY3, NY3-1 and NY3-2 ) Were 0.969,0.677,1.316 respectively. The infrared spectrum showed that the rhamnolipid produced by NY3 did not contain CC bond, while the rhamnolipid secreted by NY3-1 and NY3-2 induced by levofloxacin Contains CC unsaturated bond. Compared with ESI-MS, Rh-C10 was found in the rhamnolipid produced by the three strains, Rha-Rha-C10: 1 containing the unsaturated bond in the rhamnolipid produced by the mutagenized strain was higher .