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目的:从体内外探讨全反式视黄酸(ATRA)对人胃癌细胞转移及其相关蛋白影响.方法:胃癌细胞接种到裸鼠脾包膜,每隔两天灌胃给予ATRA 0.7 mg/kg,6周后处死裸鼠,取出所有在脾和肝形成的肿瘤,一些肿瘤被固定和包埋,另一些肿瘤保存在液氮中用于后续实验.用蛋白质印迹法测定蛋白水平;通过免疫组化显示微血管;采用粘附实验测定细胞粘附能力.结果:ATRA灌胃后,脾移植瘤和肝转移瘤受到明显抑制(50％),血管生成也受到抑制.尽管ATRA在体内外调节nm23和mts1/p16蛋白的方式不同,但高比值的nm23:mtsl/p16与低粘附性相关.ATRA在体内外诱导ICAM-1蛋白表达.结论:ATRA显著抑制移植瘤生长及其向肝转移,这一过程可能与对转移相关蛋白nm23,mstl/p16和ICAM-1的调控有关.
Objective: To investigate the effect of all-trans-retinoic acid (ATRA) on the metastasis of human gastric cancer cells and its related proteins in vitro and in vivo.Methods: The gastric cancer cells were inoculated into the splenic capsule of nude mice and intragastrically given ATRA 0.7 mg / kg , Nude mice were sacrificed 6 weeks later, all tumors formed in the spleen and liver were removed, some of the tumors were fixed and embedded, and others were stored in liquid nitrogen for subsequent experiments.Protein levels were determined by Western blotting; The adhesion ability of cells was evaluated by adhesion assay.Results: ATRA was significantly inhibited (50%) in spleen xenografts and liver metastases, and angiogenesis was also inhibited.While ATRA regulates nm23 in vitro and in vivo mts1 / p16, but high ratio of nm23: mtsl / p16 was associated with low adhesion.ATRA induced ICAM-1 protein expression in vitro and in vivo.Conclusion: ATRA can significantly inhibit the growth of xenografts and its metastasis to liver A process may be related to the regulation of metastasis-associated proteins nm23, mstl / p16 and ICAM-1.