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应用细胞外记录单位放电技术,在大鼠海马脑片上观察了白藜芦醇(resveratrol)对海马CA1区神经元放电的影响。实验结果如下:(1)在52个CA1区神经元放电单位给予白藜芦醇(0.05、0.5、5μmol/L)2min,有46个放电单位(88.5%)放电频率明显降低,且呈剂量依赖性;(2)预先用0.2mmol/L的L-glutamate灌流海马脑片,8个放电单位放电频率明显增加,表现为癫痫样放电,在此基础上灌流白藜芦醇(5μmol/L)2min,其癫痫样放电被抑制;(3)预先用L型钙通道开放剂BayK8644灌流7个海马脑片,有6个单位(85.7%)放电增加,在此基础上灌流白藜芦醇(5μmol/L)2min,其放电被抑制;(4)9个放电单位灌流一氧化氮合酶抑制剂L-NAME(NG-nitro-L-argininemethylester)50μmol/L,有7个单位(77.8%)放电明显增加,在此基础上灌流白藜芦醇(5μmol/L)2min,放电被抑制;(5)10个放电单位灌流大电导钙激活性钾通道阻断剂TEA(tetraethylammoniumchloride)1mmol/L后,有9个单位(90%)放电增加,在此基础上灌流白藜芦醇(5μmol/L)2min,8个放电单位(88.9%)放电频率明显减低。以上结果提示:白藜芦醇能抑制海马神经元自发放电以及由L-glutamate、L-NAME、BayK8644和TEA诱发的放电,可能与白藜芦醇抑制L型钙通道,减少钙内流有关;似乎与大电导钙激活性钾通道无关。
Using an extracellular recording unit discharge technique, the effect of resveratrol on neuronal firing in hippocampal CA1 region was observed on rat hippocampal slices. The experimental results are as follows:(1) Resveratrol (0.05, 0.5, 5μmol/L) was administered in 52 CA1 neuron discharge units for 2 min, and the discharge frequency of 46 discharge units (88.5%) was significantly reduced, and was dose-dependent. (2) The hippocampal slices were perfused with 0.2 mmol/L L-glutamate in advance, and the discharge frequency of 8 discharge units increased significantly, showing epileptic discharges, and on this basis, perfused resveratrol (5 μmol/L) for 2 min. The epileptiform discharge was inhibited; (3) 7 hippocampal slices were perfused with the L-type calcium channel opener BayK8644 in advance, 6 units (85.7%) of the discharge increased, and on this basis perfused resveratrol (5 μmol/l). L) 2min, the discharge was inhibited; (4) nine discharge units perfusion of nitric oxide synthase inhibitor L-NAME (NG-nitro-L-argininemethylester) 50μmol / L, 7 units (77.8%) discharge was significant Increase, on the basis of perfusion of resveratrol (5μmol/L) 2min, discharge is inhibited; (5) 10 discharge units perfused large conductance calcium-activated potassium channel blocker TEA (tetraethylammonium chloride) 1mmol/L, there are The discharge was increased in 9 units (90%), and on the basis of this, perfusion of resveratrol (5 μmol/L) for 2 min, and the discharge frequency of 8 discharge units (88.9%) was significantly reduced. The above results suggest that resveratrol can inhibit the spontaneous discharge of hippocampal neurons and the discharge induced by L-glutamate, L-NAME, BayK8644 and TEA, and may be related to resveratrol inhibiting L-type calcium channels and reducing calcium influx. It seems to have nothing to do with the large conductance calcium-activated potassium channel.