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目的通过比较哮喘患儿外周血CD4+CD25+和CD8+CD28-调节性T细胞的变化,探讨小儿哮喘的发病机制、早期防治和减少其发生的新途径。方法采用流式细胞仪测定31例哮喘患儿急性发作期和缓解期外周血中CD4+CD25+和CD8+CD28-调节性T细胞百分率;23例健康儿童作为健康对照组。采用SPSS13.0软件进行统计学分析。结果与健康对照组及缓解期比较,哮喘急性发作期患儿外周血中CD4+CD25+和CD8+CD28-调节T细胞(3.92%±1.75%;10.95%±3.46%)均明显减低(Pa<0.01);缓解期患儿CD4+CD25+与CD8+CD28-调节性T细胞水平(5.39%±2.01%;14.37%±3.92%)仍显著低于健康对照组(6.94%±2.27%;16.07%±4.28%)(Pa<0.05)。CD4+CD25+和CD8+CD28-调节性T细胞与哮喘患儿病程呈正相关(r=0.462,0.459Pa<0.001)。结论CD4+CD25+及CD8+CD28-调节性T细胞形成的免疫网络调控异常可能是导致小儿哮喘发生、发展的重要原因。CD4+CD25+和CD8+CD28-调节性T细胞水平反映呼吸道炎性反应活动情况,与病程相关。诱导调节性T细胞的表达可望成为治疗小儿哮喘的一个新途径。
Objective To compare the changes of CD4 + CD25 + and CD8 + CD28- regulatory T cells in peripheral blood of children with asthma to explore the pathogenesis of early childhood asthma, early prevention and treatment and to reduce the occurrence of new ways. Methods The percentage of CD4 + CD25 + and CD8 + CD28 + regulatory T cells in peripheral blood of 31 asthmatic children during acute exacerbation and remission were determined by flow cytometry. Twenty-three healthy children served as healthy controls. SPSS13.0 software was used for statistical analysis. Results Compared with healthy control group and remission stage, CD4 + CD25 + and CD8 + CD28- regulatory T cells (3.92% ± 1.75%; 10.95% ± 3.46%) were significantly decreased in peripheral blood of children with acute exacerbation of asthma ). The levels of CD4 + CD25 + and CD8 + CD28- regulatory T cells in children with remission (5.39% ± 2.01%; 14.37% ± 3.92%) were still significantly lower than those in healthy controls (6.94% ± 2.27%; 16.07% ± 4.28 %) (Pa <0.05). The duration of CD4 + CD25 + and CD8 + CD28-regulatory T cells was positively correlated with the course of asthma (r = 0.462, 0.459Pa <0.001). Conclusion Abnormal regulation of immune network formed by CD4 + CD25 + and CD8 + CD28- regulatory T cells may be the important reason for the occurrence and development of asthma in children. CD4 + CD25 + and CD8 + CD28- regulatory T-cell levels reflect respiratory inflammatory response activity, and duration. Induced the expression of regulatory T cells is expected to become a new way to treat pediatric asthma.