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选择香菇的野生株 （ Ｑ） 与栽培株苏香两种孢子单核体 （ Ａ１ Ｂ１， Ａ２ Ｂ２） 进行完全亲和双单杂交， 以拮抗试验辅以液体出菇试验初步鉴定双单杂交后代， 并以 Ｒ Ａ Ｐ Ｄ技术 （随机扩增的多态性 Ｄ Ｎ Ａ 技术） 对杂交后代进行检测。每个杂交组合只得到一个杂交后代， 说明在完全亲和双单杂交供体中存在先导核。运用原生质体单核化与交配型鉴定技术对杂交后代中来自供体的先导核进行了追踪研究。结果表明， 受体交配型不同时， 其先导核也不同。在 （ Ａ３ Ｂ３＋ Ａ４ Ｂ４） × Ａ１ Ｂ１双单杂交中先导核为 Ａ３ Ｂ３ 核， 在 （ Ａ３ Ｂ３＋ Ａ４ Ｂ４） × Ａ２ Ｂ２ 双单杂交中先导核为 Ａ４ Ｂ４ 核。
The wild-type (Q) of Mushroom and the single-hybrid of the two kinds of sporophytes (A1 B1, A2 B2) of the cultivated strain Su-Xiang were selected for complete affinity double-single hybridization. Offspring, and hybrid progeny were tested with the RAPD technique (Randomly Amplified Polymorphic D N A Technique). Only one hybrid progeny is obtained for each cross combination, indicating that there is a preponderance nucleus in a fully compatible double-single hybrid donor. The precursor nuclei from the donor in the progeny of the hybrids were traced using protoplast and nucleoplasmic identification techniques. The results showed that when the receptor mating type is different, its leading nucleus is also different. The leading nucleus was A3 B3 nucleus in (A3 B3 + A4 B4) × A1 B1 doublemonth hybridization and A4 B4 nucleus in (A3 B3 + A4 B4) × A2 B2 doublesingle hybridization.