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生物样品阿霉素除用生物检定、荧光法、同位素法检测外,本文报道可采用萤光检测器进行高效液相层析(HPLC)。本法因不受其它物质干扰故最常采用。日立635A 型 HPLC 仪,附设650-10S 型高灵敏度萤光检测器;填充剂 Lorbax Sil(5μm)填充于150×4.6mm(内径)的不锈钢柱内,流动相为含3.8％醋酸钠(pH4.5)的异丙醇液,流速:1.0ml/分;于470nm 激发波长和585nm 发射波长下测定,内标为柔红霉素。阿霉素脂质体的制备和测定:将蛋卵磷脂(EL)-胆甾醇(Chol)(中性,克分子比7∶2)或 EL-Chol-联十六烷基磷酸盐(负电性,克分子比7∶2∶1)或 EL-Chol-十八酰胺(正电性,克分子比7∶2∶1)置圆底烧并中,加
In addition to biological samples of doxorubicin bioassay, fluorescence, isotope detection, the paper reported fluorescence detector can be used for high performance liquid chromatography (HPLC). This law is most commonly used because it is not disturbed by other substances. The Hitachi 635A HPLC instrument was equipped with a 650-10S high-sensitivity fluorescent detector. The filler Lorbax Sil (5 μm) was packed in a stainless steel column of 150 × 4.6 mm (inner diameter) with a mobile phase of 3.8% sodium acetate pH4. 5) isopropanol solution, flow rate: 1.0ml / min; at an excitation wavelength of 470nm and an emission wavelength of 585nm, the internal standard is daunorubicin. Preparation and determination of doxorubicin liposomes: EL-Choles (neutral, molar ratio 7: 2) or EL-Chol-cetyl phosphate , Molar ratio of 7: 2: 1) or EL-Chol-octadecanamide (positive, molar ratio of 7: 2: 1)