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为了探讨丁酸钠对人肝癌细胞各种酶活性的影响，采用丁酸钠处理Ｂｅｌ－７４０２人肝癌细胞，测定其γ－谷氨酰转肽酶（γ－ＧＴ）、酪氨酸－α－酮戊二酸转氨酶（ＴＡＴ）、醛缩酶（ＡＬＤ）的比活力和细胞增殖速度。结果显示，２．５ｍｍｏｌ／Ｌ和５．０ｍｍｏｌ／Ｌ的丁酸钠能明显抑制人肝癌细胞增殖，并使肝癌标志酶γ－ＧＴ活性及反映肝细胞恶变或损伤的ＡＬＤ活性明显降低（Ｐ＜０．０１，Ｐ＜０．０５），而使反映肝细胞分化的ＴＡＴ活性明显升高（Ｐ＜０．０１，Ｐ＜０．０５）。结果表明，丁酸钠能引起人肝癌细胞γ－ＧＴ、ＴＡＴ、ＡＬＤ明显改变，提示这几种酶可作为肝癌细胞分化诱导的酶学指标
To investigate the effect of sodium butyrate on the activity of various enzymes in human hepatocellular carcinoma cells, Bel-7402 human hepatocellular carcinoma cells were treated with sodium butyrate and the activities of γ-glutamyl transpeptidase (γ-GT), tyrosine- Ketoglutarate transaminase (TAT), aldolase (ALD) specific activity and cell proliferation rate. The results showed that 2.5mmol / L sodium butyrate and 5.0mmol / L sodium butyrate could significantly inhibit the proliferation of human hepatocellular carcinoma cells, and markedly decreased the activity of hepatic cancer marker γ-GT and the ALD activity reflecting hepatocellular malignant transformation or injury (P < 0.01, P <0.05), while the TAT activity reflecting hepatocyte differentiation was significantly increased (P <0.01, P <0.05). The results showed that sodium butyrate can cause significant changes of γ-GT, TAT and ALD in human hepatoma cells, suggesting that these enzymes could be used as indicators of differentiation induced by hepatoma cells