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目的探讨脑胶质瘤相关癌基因(glioma-associated oncogene1,Gli1)和骨桥蛋白(osteopontin,OPN)在结直肠癌组织中的表达情况及临床意义。方法应用免疫组化SP法检测Gli1和OPN蛋白在76例结直肠癌组织,23例正常结直肠组织中的表达情况,分析Gli1和OPN与结直肠癌患者的临床病理因素之间的关系以及二者之间表达的相关性。结果 (1)Gli1在结直肠癌组织和正常结肠组织中表达的阳性率分别为43.4%(33/76)和13.0%(3/23),两者差异具有统计学意义(P<0.05);OPN在结直肠癌组织和正常结肠组织中表达的阳性率分别为51.3%(39/76)和17.4%(4/23),差异有统计学意义(P<0.05)。(2)Spearman等级相关分析提示,Gli1和OPN在结直肠癌组织间的表达呈正相关(r=0.312,P<0.01)。结论 Gli1和OPN参与了结直肠癌的发生发展过程,二者之间可能起协同作用,联合检测Gli1和OPN对于评估结直肠癌的生物学特性和判断预后具有一定价值。
Objective To investigate the expression and clinical significance of glioma-associated oncogene1 (Gli1) and osteopontin (OPN) in colorectal cancer. Methods Immunohistochemical SP method was used to detect the expression of Gli1 and OPN protein in 76 cases of colorectal cancer tissue and 23 cases of normal colorectal tissue. The relationship between Gli1, OPN and clinicopathological factors in patients with colorectal cancer was analyzed. Correlation between the expression of. Results (1) The positive rates of Gli1 expression in colorectal cancer tissues and normal colon tissues were 43.4% (33/76) and 13.0% (3/23), respectively, with statistical significance (P <0.05). The positive rates of OPN expression in colorectal cancer tissues and normal colon tissues were 51.3% (39/76) and 17.4% (4/23), respectively, with statistical significance (P <0.05). (2) Spearman rank correlation analysis showed that the expression of Gli1 and OPN in colorectal cancer tissues was positively correlated (r = 0.312, P <0.01). Conclusions Gli1 and OPN are involved in the development and progression of colorectal cancer. The two may play synergistic roles. Combined detection of Gli1 and OPN is of great value in assessing the biological characteristics and prognosis of colorectal cancer.