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采用生长速度法测定了番茄灰叶斑病原菌在不同温度、光照下的萌发生长情况和常用化学药剂的防治效果,采用对峙实验筛选了防控番茄灰叶斑病的生防菌株。结果表明:番茄灰叶斑病菌最佳的生长温度为30℃左右,光照对其影响不显著;A药剂抑制效果最好,在20℃、25℃抑菌率达100%;30℃在A1浓度下菌丝第四天萌发,在A2浓度下第三天萌发。匍枝根霉、黑根霉和拟康氏木霉对番茄灰叶斑病原菌的抑菌率都在80%以上,其中匍枝根霉的抑菌率达87.68%;拟康氏木霉对峙病原菌时病原菌落萎缩、交界处病原菌丝消解。
Growth and growth method was used to determine the germination and growth of tomato gray leaf spot pathogens at different temperatures and light and the control effect of common chemical agents. The biocontrol strains against tomato gray leaf spot were screened by confrontation experiment. The results showed that the optimum growth temperature of C. citri was about 30 ℃, and the effect of light on it was not significant. The best inhibiting effect of agent A was that the antibacterial rate was 100% at 20 ℃ and 25 ℃, The next day mycelium germination, germination in the third day at the A2 concentration. Rhizopus stolonifer and Trichoderma koningii were more than 80%, and the inhibitory rate of Rhizopus stolonifer reached 87.68%. Strains of Trichoderma koningii against Staphylococcus aureus Pathogen colony shrinks, the junction of pathogenic mycelia digestion.