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目的：研究利用siRNA方法基因沉默人结肠癌SW480细胞dcr3基因的表达，并观察受基因沉默后人结肠癌SW480细胞体外增殖情况。方法：利用脂质体将siRNA转染进入人结肠癌SW480细胞系，在细胞内形成小干扰双链RNA，识别并降解dcr3mRNA。通过MTT法检测人结肠癌SW480细胞的增殖抑制情况。结果：siRNA方法基因沉默人结肠癌SW480细胞dcr3基因后，其细胞增殖率降低（P＜0.05）结论：siRNA方法基因沉默人结肠癌SW480细胞dcr3基因的表达后，细胞增殖率降低。“,”AIM:To study relationship between depressed dcr3 gene expression and growth of human colonic cancer SW480 cells . METHODS: Using siRNA method,we constructed a smal interfering siRNA, then transfected it into human colonic cancer SW480 cells by liposome. The expression level of dcr3 mRNA was detected by realtime RT-PCR after they were incubated for 24h.Extract EGFR protein form SW480 cellafter transfection.Study dcr3 expression by The cellcycles and cellproliferations of SW480 cellwere measured with MTT method after transfection.RESULTS: The MTT assay results showed that cellsurvival rate of SW480 was demonstrated and livingness of cells was declined CONCLUSION: Dcr3-RNAi down-regulates the expression of EGFR mRNA in SW480 colonic cancer cells, which leads to inhibition of cellgrowth and enhancement of cellapoptosis.