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目的 :探讨早期生长反应基因 1(EGR 1)对肝癌和食管癌细胞的生长是否有抑制作用。方法 :用脂质体技术将EGR 1真核表达载体转染于无EGR 1转录、表达的肝癌及食管癌细胞株中 ,观察外源高表达的EGR 1对二癌细胞生长、细胞周期、克隆形成及致瘤性的影响 ,以空白载体为对照。结果 :转染EGR 1的HHCC细胞株 (肝癌 )和ECa10 9细胞株 (食管癌 )的生长速率较对照组明显减慢 ,S期细胞比例分别减少 45 5 %和 34 1% ,平板克隆形成率分别下降 46 6 %和 41 8% ,致瘤性分别下降 80 4%和 72 6 %。但SMMC 772 1细胞株 (肝癌 )上述各项指标均与对照组无明显差别。结论 :EGR 1对肝癌HHCC细胞株和食管癌ECa10 9细胞株的生长及恶性表型有明显抑制作用 ,而对肝癌SMMC 772 1细胞株则无抑制作用
Objective: To investigate whether early growth response gene 1 (EGR 1) can inhibit the growth of liver cancer and esophageal cancer cells. METHODS: Eukaryotic expression vector of EGR 1 was transfected into hepatocellular carcinoma and esophageal cancer cell lines without EGR 1 expression by liposome technology. The growth, cell cycle, and cloning of exogenous high expression of EGR 1 on cancer cells were observed. The formation and tumorigenic effects were compared with the blank vector. Results: The growth rates of HHCC cell line (liver cancer) and ECa109 cell line (esophageal cancer) transfected with EGR 1 were significantly slower than those of the control group. The percentage of S phase cells was reduced by 45.5% and 34.1%, respectively. They decreased 466% and 41% respectively, and the tumorigenicity decreased by 804% and 72.6%, respectively. However, the SMMC 772 1 cell line (liver cancer) was not significantly different from the control group. Conclusion : EGR 1 can significantly inhibit the growth and malignant phenotype of HHCC cell line and esophageal carcinoma ECa 10 9 cell line, while it has no inhibitory effect on SMMC 772 1 cell line.