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目的了解胃癌细胞ＳＧＣ７９０１及其耐药细胞ＳＧＣ７９０１／ＶＣＲ中ＨＳＰ９０β蛋白的表达，与化疗药物敏感性的关系及降低细胞ＨＳＰ９０β蛋白的表达是否影响化疗药物的敏感性。方法用免疫组化法检测胃癌细胞ＳＧＣ７９０１及其耐药细胞ＳＧＣ７９０１／ＶＣＲ中ＨＳＰ９０β蛋白的表达。ＭＴＴ法测定ＳＧＣ７９０１、ＳＧＣ７９０１／ＶＣＲ细胞及其相应的ＨＳＰ９０β蛋白低表达细胞ＡＨ－ＳＧＣ７９０１和ＡＨ－ＳＧＣ７９０１／ＶＣＲ对化疗药物的敏感性，流式细胞仪测定细胞内药物含量。结果ＳＧＣ７９０１／ＶＣＲ细胞ＨＳＰ９０β蛋白的表达高于ＳＧＣ７９０１细胞。ＳＧＣ７９０１／ＶＣＲ细胞对ＡＤＭ、ＶＣＲ、ＭＭＣ和ＣＴＸ的ＩＣ５０分别为０．３９８、１．２５、０．１９９和３ ９８１．０７μｇ／ｍｌ。ＳＧＣ７９０１对ＡＤＭ、ＶＣＲ、ＭＭＣ及ＣＴＸ的ＩＣ５０分别为１．５８×１０－６、１．９９×１０－３、３．１×１０－２及６３０μｇ／ｍｌ。ＳＧＣ７９０１／ＶＣＲ相对于ＳＧＣ７９０１细胞对ＡＤＭ、ＶＣＲ、ＭＭＣ和ＣＴＸ的耐药指数分别为２．５２×１０５、６．２８×１０２、６．４２和６．３２。ＡＨ－ＳＧＣ７９０１和ＡＨ－ＳＧＣ７９０１／ＶＣＲ对化疗
Objective To understand the expression of HSP90β protein in gastric cancer cell line SGC7901 and its resistant cell SGC7901/VCR, and to determine whether the expression of HSP90β protein affects the sensitivity of chemotherapeutic drugs. Methods The expression of HSP90β protein in gastric cancer cell SGC7901 and its resistant cell SGC7901/VCR was detected by immunohistochemistry. The sensitivities of SGC7901, SGC7901/VCR cells and their corresponding HSP90β low expressing cells AH-SGC7901 and AH-SGC7901/VCR to chemotherapeutic drugs were measured by MTT assay. The intracellular drug content was measured by flow cytometry. Results The expression of HSP90β protein in SGC7901/VCR cells was higher than that in SGC7901 cells. The IC50 of SGC7901/VCR cells against ADM, VCR, MMC, and CTX were 0.398, 1.25, 0.199, and 3981.07 μg/ml, respectively. The IC50 of SGC7901 against ADM, VCR, MMC, and CTX were 1.58×10-6, 1.99×10-3, 3.1×10-2, and 630 μg/ml, respectively. The resistance indices of SGC7901/VCR to ADM, VCR, MMC, and CTX relative to SGC7901 cells were 2.52×105, 6.28×102, 6.42, and 6.32, respectively. AH-SGC7901 and AH-SGC7901/VCR for Chemotherapy