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在实验室建立了人蚤实验种群,运用了交叉传代法以保持种群的遗传稳定性;同时运用RAPD技术对人蚤实验种群遗传结构的时间变化作了监测,以确认遗传结构的稳定和传代方法的有效性。结果表明,在5个的时间内,人蚤实验种群的遗传结构没有发生大的变化,种群内遗传距离分别为0.2088±0.0517和0.2140±0.0787。说明交叉传代法对保持实验种群的遗传稳定性是有效的,RAPD技术在遗传稳定性监测方面的运用也是可行的。同时对RAPD技术在这方面运用时的一些问题作了讨论。
The experimental population of human fleas was established in the laboratory, and the genetic stability of the population was maintained by the cross-passage method. Meanwhile, the temporal variation of the genetic structure of the experimental population in the flea population was monitored by RAPD to confirm the stability of the genetic structure and the passage method Effectiveness. The results showed that there was no significant change in the genetic structure of the flea population within 5 days, and the intraspecific genetic distances were 0.2088 ± 0.0517 and 0.2140 ± 0.0787, respectively. It shows that cross-passage method is effective in maintaining the genetic stability of experimental population, and the application of RAPD in genetic stability monitoring is also feasible. At the same time, some problems of using RAPD technology in this aspect are discussed.