论文部分内容阅读
目的:建立同时测定人参三七颗粒中人参皂苷Rg_1、Re、Rb_1和三七皂苷R_1含量的方法。方法:采用HPLC法,色谱柱为Sunfire C_(18)(150mm×4.6mm,5μm)分析柱.流动相以乙腈-水梯度洗脱;检测波长为203nm;柱温30℃;流速1.0ml·min~(-1)。结果:人参皂苷Rg_1,Re,Rb_1和三七皂苷R_1之间有较好的分离度,4种成分在线性范围內与峰面积之间线性关系良好,人参皂苷Rg_1、Re、Rb_1和三七皂苷R_1加样回收率分别为99.83%,97.84%,98.43%,97.34%,RSD分别为2.08%,1.66%,1.73%和1.42%(n:5)。结论:本方法可同时测定人参三七颗粒中的人参皂苷Rg_1,Re,Rb_1和三七皂苷R_1含量。
Objective: To establish a method for simultaneous determination of ginsenoside Rg_1, Re, Rb_1 and notoginsenoside R_1 in Panax Notoginseng Granules. Methods: The analytical column was Sunfire C 18 (150mm × 4.6mm, 5μm) using HPLC method.The mobile phase was eluted with acetonitrile-water gradient.The detection wavelength was at 203nm.The column temperature was 30 ℃ .The flow rate was 1.0ml · min ~ (-1). Results: Ginsenosides Rg_1, Re, Rb_1 and notoginsenoside R_1 had good resolution. There was a good linear relationship between the four components in the linear range and the peak area. Ginsenosides Rg_1, Re, Rb_1 and notoginsenoside The recoveries of R_1 were 99.83%, 97.84%, 98.43% and 97.34%, respectively. The RSDs were 2.08%, 1.66%, 1.73% and 1.42%, respectively. Conclusion: This method can simultaneously determine ginsenoside Rg_1, Re, Rb_1 and notoginsenoside R_1 in Panax notoginseng.