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目的:比较荧光偏振免疫法(FPIA)和化学发光微粒子免疫法(CMIA)监测全血环孢霉素A(CsA)浓度结果的相关性。方法:收集服用CsA患者的稳态浓度全血样本,分别用FPIA法和CMIA法进行测定,考察2种方法的相关程度;进一步将浓度结果分为低浓度组和高浓度组进行相关性研究。结果:2种方法测定全血CsA浓度结果r=0.992具有显著性差异(P<0.05),CMIA法测定结果偏低。低浓度组结果r=0.961具有显著性差异(P<0.05);高浓度组结果r=0.992亦具有显著性差异(P<0.05)。结论:CMIA法作为新的全血CsA浓度监测方法,测定结果比FPIA法要显著降低,但监测数据更为准确,需要向医师和患者说明。
OBJECTIVE: To compare the results of the fluorometric polarization immunoassay (FPIA) and chemiluminescence microparticle immunoassay (CMIA) to monitor the concentration of cyclosporin A (CsA) in whole blood. Methods: The steady-state whole blood samples collected from patients with CsA were collected and measured by FPIA and CMIA respectively. The correlation between the two methods was also investigated. The concentration was divided into low concentration group and high concentration group. Results: There were significant differences (r = 0.992) between the two methods in determining the CsA concentration in whole blood (P <0.05). The results of CMIA assay were low. There was significant difference (r = 0.961, r = 0.992, r = 0.992, P <0.05) in high concentration group. Conclusion: As a new method of monitoring whole blood CsA concentration, CMIA method has a significantly lower result than FPIA method, but the monitoring data is more accurate and needs to be explained to physicians and patients.