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目的检测头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)标本,寻找与HNSCC转移有高度相关性的基因。方法采用比较基因组杂交芯片技术,分别检测80例临床HNSCC标本,包括舌癌17例,口腔癌13例,口咽癌14例,下咽癌36例。结果比较基因组杂交CGH检测结果示同未发生转移的HNSCC组织标本相比,发生转移的HNSCC组织标本内,基因扩增有显著性差异的位置有1q、4q、7q、11q、Xq,基因缺失有显著性差异的位置有5p、8p、9p、11q、13q、17p。Cox单因素分析发现这些差异基因主要涉及到转录调控和信号传导等功能分类。结论 HNSCC标本中存在与HNSCC转移相关的扩增基因和缺失基因。
Objective To detect head and neck squamous cell carcinoma (HNSCC) specimens and to find out the genes highly correlated with the metastasis of HNSCC. Methods 80 cases of clinical HNSCC specimens were detected by comparative genomic hybridization chip technique, including 17 cases of tongue cancer, 13 cases of oral cancer, 14 cases of oropharyngeal carcinoma and 36 cases of hypopharyngeal carcinoma. Results Compared with the HNSCC tissue samples without metastasis, the results of comparative CGH showed that there were 1q, 4q, 7q, 11q and Xq in the HNSCC tissue samples with metastasis, the gene deletion was Significant differences in the location of 5p, 8p, 9p, 11q, 13q, 17p. Cox univariate analysis found that these differential genes mainly involved in transcriptional regulation and signaling and other functional classification. Conclusions The HNSCC specimens contain amplification and deletion genes related to HNSCC metastasis.