论文部分内容阅读
目的探讨乳腺癌患者骨髓中人乳腺珠蛋白(hMAM)mRNA的表达及其临床意义。方法应用巢式RT-PCR技术,同时检测75例乳腺癌患者、15例乳腺良性病变患者和8例健康人骨髓中hMAM mRNA的表达,分析hMAM mRNA表达与临床病理因素、Ki67、p53和血管内皮生长因子(VEGF)的关系。结果RT-PCR检测的敏感度达到10-6。75例乳腺癌患者中,21例检测出hMAM mRNA阳性表达,阳性表达率为28．0％。hMAM mRNA阳性表达与乳腺癌腋窝淋巴结转移和PR状况有关(P<0．05),与年龄、肿瘤大小、临床分期和ER状况无关(P>0．05);与乳腺癌组织Ki67表达呈正相关(x2=4．936,P=0．026)。乳腺良性病变患者和健康人骨髓中,未检测到hMAM mRNA表达。结论应用RT-PCR方法检测乳腺癌骨髓中hMAM mRNA的表达,敏感度高、特异性好。hMAM mRNA可作为检测乳腺癌患者骨髓中播散肿瘤细胞的分子指标之一,可为乳腺癌患者的治疗和预后判断提供帮助。
Objective To investigate the expression of human mammaglobin (hMAM) mRNA in bone marrow of breast cancer patients and its clinical significance. METHODS: Nested RT-PCR was used to detect the expression of hMAM mRNA in bone marrow of 75 patients with breast cancer, 15 patients with benign breast disease, and 8 healthy controls. The expression of hMAM mRNA and clinicopathological factors, Ki67, p53 and vascular endothelium were analyzed. Growth factor (VEGF) relationship. Results The sensitivity of RT-PCR detection was 10-6. In 75 breast cancer patients, hMAM mRNA expression was detected in 21 cases, and the positive expression rate was 28.0%. The positive expression of hMAM mRNA was associated with axillary lymph node metastasis and PR status (P<0.05), but not with age, tumor size, clinical stage, and ER status (P>0.05); it was positively correlated with Ki67 expression in breast cancer tissues. (x2=4.936, P=0.026). No hMAM mRNA expression was detected in benign breast lesions and healthy human bone marrow. Conclusion The RT-PCR method was used to detect the expression of hMAM mRNA in bone marrow of breast cancer with high sensitivity and specificity. hMAM mRNA can be used as one of the molecular markers for detecting disseminated tumor cells in the bone marrow of breast cancer patients, and can provide help for the treatment and prognosis of breast cancer patients.