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以转抑制衰老有关的异戊烯基转移酶 (IPT)基因株系GC 1、携带抗白叶枯病基因Xa2 3的“CBB2 3”和抗稻瘟病水稻品种“合系 15号”为供体 ,采用分子标记辅助选择与生物学鉴定相结合的方法 ,将IPT基因与Xa2 3及抗稻瘟病基因进行聚合。在 3个复交组合中获得了 17株聚合IPT基因与Xa2 3基因的植株 ,用这些植株与两系杂交稻亲本 9311、E32、培矮 6 4S及W9834S进行杂交和回交 ,经PCR分子检测和抗白叶枯病、抗稻瘟病鉴定和细胞分裂素含量的测定 ,最终在 4个BC1回交组合“(9311/ / /合系 15 /CBB2 3∥GC 1)× 9311”、“(E32 / / /合系15 /CBB2 3∥GC 1)×E32”、“(培矮 6 4S/ / /合系 15 /CBB2 3∥GC 1)×培矮 6 4S”和“(GC 1/CBB2 3/ /W 9834S/合系 15 )×W 98 34S”中获得了 17株携带IPT基因与Xa2 3基因的BC1F1植株 ,这些植株对来自北方稻区 2 1个稻瘟病菌系全部表现为抗。携带IPT基因的抗病植株再与杂交稻亲本进行回交 ,在 2个BC2 回交组合“[(9311///合系 15 /CBB2 3∥GC 1)× 9311]× 9311”和“[(E32 ///合系15 /CBB2 3∥GC 1)×E32 ]×E32”中获得了 7株携带IPT基因与Xa2 3基因的植株 ,这些植株再经过 1～ 2代回交和自交 ,即可用于杂交稻育种
In order to suppress the senescence-related isopentenyltransferase (IPT) gene line GC 1, CBB 3 carrying the bacterial resistance gene Xa 2 3 and the rice variety “Cohort 15” , The IPT gene was polymerized with Xa2 3 and blast resistance genes by molecular marker-assisted selection and biological identification. Seven plants with IPT and Xa2 3 genes were obtained from the three multiple crossing combinations. The plants were crossed and backcrossed with two-line hybrid rice parents 9311, E32, Pei’ai 64S and W9834S, Bacterial blight resistance, blast resistance identification and cytokinin content determination were carried out in four BC1 backcross combinations (9311 / / line 15 / CBB2 3 // GC1 × 9311 “,” (E32 / / / Co-line 15 / CBB2 3 // GC 1) × E32 “,” Pevee 6 4S / / Co-line 15 / CBB2 3 //GC 1 × Pegasi 4 4S and GC 1 / CBB2 3 / Seventeen BC1F1 plants carrying the IPT gene and the Xa2 3 gene were obtained from the W 9834S / line 15 × W 98 34S. All of these plants showed resistance to 21 blast fungus strains from the northern rice area. The resistant plants carrying IPT gene were backcrossed with the parents of the hybrid rice and backcrossed in the two BC2 backcross combinations of “[(9311 /// co-15 / CBB2 3 // GC1) × 9311” × 9311 " Seven plants carrying IPT gene and Xa2 3 gene were obtained from E32 /// Affinity 15 / CBB2 3∥GC 1) × E32] × E32. These plants were backcrossed and selfed after one to two generations, that is, Can be used for hybrid rice breeding