Direct Electrochemistry of Horseradish Peroxidase Embedded in Nano-Fe_3O_4 Matrix on Paraffin Impreg

• 作 者: 龚静鸣 林祥钦
• 机 构: Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China,D
• 出 处: Chinese Journal of Chemistry
• 发表日期: 2003年07期
• 关键词: copolymer voltammetry demon graphite coated peroxidase acrylic amide biosensors

Fe_3O_4particles coated with acrylic copolymer (ACP) of about5—8 nm in diameter were synthesized and used for immobiliza-tion of horseradish peroxidase (HRP). Direct electrochemistryof HRP embedded in the nanosized Fe_3O_4 solid matrix modifiedparaffin impregnated graphite electrode (PIGE) was achieved,which is related to the heme Fe(Ⅲ)/Fe(Ⅱ) conversion ofHRP. Cyclic voltammetry gave a pair of reproducible and well-defined redox peaks at about E_m of-0.295 V vs. SCE. Thestandard rate constant k_s was determined as 2.7 s~(-1). It demon-

Fe_3O_4particles coated with acrylic copolymer (ACP) of about 5-8 nm in diameter were synthesized and used for immobiliza tion of horseradish peroxidase (HRP). Direct electrochemistry of HRP embedded in the nanosized Fe_3O_4 solid matrix modified paraffin impregnated graphite electrode (PIGE) was achieved, which is related to the heme Fe (III) / Fe (II) conversion ofHRP. Cyclic voltammetry gave a pair of reproducible and well-defined redox peaks at about E_m of -0.295 V vs. SCE. The standard rate constant k_s was determined as 2.7 s ~ (-1). It demon-