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[目的]研究900 MHz、最大功率密度为10.644μW/cm2的手机射频辐射对体外细胞DNA损伤和病毒癌基因EB病毒早期抗原(EBV-EA)的激活作用。[方法](1)将NIH/3T3细胞分别暴露在手机辐射下0、1、2、4、6、8、12 h,并分别设定相应的正常对照组,用彗星电泳检测细胞DNA受损程度。(2)将Raji细胞分为A辐照(-)TPA(-)、B辐照(-)TPA(+)、C辐照(+)TPA(-)、D辐照(+)TPA(+)4组,接受手机辐照,强度为4 h/d,实验持续4周;每周结束时,B和D两组细胞分别使用促癌剂12-0-十四烷酰佛波醇-13-乙酸酯(TPA)1 ng/mL处理48 h。用免疫细胞化学检测各组细胞中EBV-EA的阳性表达情况。[结果]当NIH/3T3细胞辐照2 h后,与对照组相比细胞Olive尾距有明显差异(P<0.05),但在随后的2~12 h内的各个检测时间点上,Olive尾距并没有随着时间的增加而增加(P>0.05);Raji细胞在实验4周后,辐照C、D组细胞的EBV-EA阳性表达率较A组明显增加(P<0.05),并且在加入促癌剂TPA的D组,阳性表达率更加明显(P<0.01)。[结论]900 MHz手机射频辐射会对细胞DNA产生一定的损伤,并且能够激活EBV-EA的表达。
[Objective] To investigate the effects of radiofrequency radiation of cell phone with maximum power density of 10.644μW / cm2 at 900 MHz on cell DNA damage and EBV-EBV activation in vitro. [Methods] (1) NIH / 3T3 cells were exposed to cell phone radiation for 0, 1, 2, 4, 6, 8 and 12 h, respectively. Corresponding normal control group was set up. DNA damage was detected by comet assay degree. (2) Raji cells were divided into A (-) TPA (-), B irradiation (-) TPA (+), C irradiation (+) TPA ) 4 groups, receiving cell phone radiation, the intensity of 4 h / d, the experiment lasted for 4 weeks; at the end of each week, B and D cells were treated with the promotion agent 12-0-tetradecanoylphorbol-13 - acetate (TPA) 1 ng / mL for 48 h. Immunocytochemistry was used to detect the positive expression of EBV-EA in each group of cells. [Result] When the NIH / 3T3 cells were irradiated for 2 h, there was a significant difference (P <0.05) in the Olive tail distance of the cells compared with the control group, but at each detection time point within 2-12 h, (P> 0.05). After 4 weeks, the positive rate of EBV-EA expression in Raji cells was significantly higher than that of A group (P <0.05), and The positive expression rate in group D with promoting agent TPA was more obvious (P <0.01). [Conclusion] The radio frequency radiation of 900 MHz cell phone could damage the DNA of cells and activate the expression of EBV-EA.