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目的研究体外受精-胚胎移植(IVF-ET)中短时受精对高龄女性助孕结局的影响。方法回顾性分析行IVFET的高龄女性(年龄≥35岁),共227个周期,按精卵混合时间分为两组:短时受精组541个卵子行短时受精(精卵混合2 h),常规受精组1 677个卵子行常规受精(精卵混合约16~20 h)。比较两组女性的受精率、胚胎质量及妊娠率,并进行统计分析,评估短时受精对高龄女性助孕结局的影响。结果短时受精组与常规受精组患者的基本情况、完全不受精率和受精率<30%的周期率比较差异均无统计学意义(P>0.05)。短时受精组的优质胚胎率为87.19%(177/203),显著高于正常受精组的61.35%(600/978),差异有统计学意义(P<0.05)。短时受精组的种植率及临床妊娠率均稍高于常规受精组,但差异均无统计学意义(P>0.05)。结论患者年龄≥35岁时,IVF-ET短时受精可明显提高优质胚胎率,改善助孕结局。
Objective To investigate the effect of short-term fertilization (IVF-ET) on fertilization outcomes in elderly women. Methods A retrospective analysis of elderly women (≥35 years of age) who underwent IVFET was performed in 227 cycles. According to the time of sperm-egg mixing, 541 eggs were fertilized for short time (sperm-egg mixed for 2 h) Conventional fertilization group 1 677 eggs routinely fertilized (sperm egg hybrid about 16 ~ 20 h). The fertilization rate, embryo quality and pregnancy rate were compared between the two groups, and statistical analysis was conducted to evaluate the effect of short-term fertilization on the pregnancy outcome in older women. Results There was no significant difference in the basic conditions of patients with short fertilization and those with conventional fertilization (P <0.05). The rate of high quality embryos in short-term fertilization group was 87.19% (177/203), which was significantly higher than 61.35% (600/978) in normal fertilization group (P <0.05). Short-term fertilization group implantation rate and clinical pregnancy rate were slightly higher than the conventional fertilization group, but the difference was not statistically significant (P> 0.05). Conclusions IVF-ET short-term fertilization can significantly improve the rate of high-quality embryos and improve the pregnancy outcomes when the patients are over 35 years of age.