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以‘克新一号’品种为试验材料,采用组织培养方法,研究了0、5、10、15、20 mmol·L-1CaCl2对0、25、50、75mmol·L-1NaCl胁迫下马铃薯脱毒苗氮代谢的影响。结果显示,随着NaCl胁迫浓度的增加,马铃薯脱毒苗叶片硝态氮含量先升高后降低,氨态氮含量持续升高,全氮和可溶性蛋白含量以及硝酸还原酶(nitrate reductase,NR)和谷氨酰胺合成酶(glutamine synthetase,GS)活性持续下降。在0、25、50 mmol·L-1NaCl胁迫下添加10 mmol·L-1CaCl2,以及在75 mmol·L-1NaCl胁迫下添加15 mmol·L-1CaCl2,可有效缓解盐胁迫对NR和GS的抑制作用,显著降低硝态氮和氨态氮含量,增强氮素同化作用,显著增加全氮和可溶性蛋白含量,改善植株氮素营养,减轻NaCl胁迫对植株的伤害。
Taking ’Kexin No.1’ as tested material, the tissue culture method was used to study the effects of 0, 5, 10, 15 and 20 mmol·L-1 CaCl2 on detoxification of potato under 0, 25, 50 and 75 mmol·L- Effect of seedling nitrogen metabolism. The results showed that with the increase of NaCl stress concentration, the nitrate nitrogen content in leaves of virus-free potato seedlings increased first and then decreased, the content of ammonia nitrogen continued to increase, the content of total nitrogen and soluble protein and nitrate reductase (NR) And glutamine synthetase (GS) activity continued to decline. Adding 10 mmol·L-1 CaCl2 under 0, 25, 50 mmol·L-1 NaCl stress and 15 mmol·L-1 CaCl2 under 75 mmol·L-1 NaCl stress can effectively alleviate the inhibition of NR and GS by salt stress Which significantly reduced nitrate nitrogen and ammonia nitrogen content, enhanced nitrogen assimilation, significantly increased total nitrogen and soluble protein content, improved plant nitrogen nutrition, and alleviated plant damage caused by NaCl stress.