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为了建立寒地粳稻成熟胚的高频再生体系,以寒地粳稻空育131和东农425为试验材料进行成熟胚培养,研究CuSO4和烯效唑的单独和组合使用对水稻成熟胚培养的影响。结果表明:在培养基中单独添加15.0μmol/L的Cu2+或添加0.50 mg/L的烯效唑有助于寒地粳稻成熟胚的诱导继代和分化;在诱导和分化培养基中同时添加CuSO4和烯效唑时,空育131在CuSO4浓度为20.0μmol/L且烯效唑浓度为1.00 mg/L时,愈伤组织的诱导率和分化率都达到最高;CuSO4浓度为10.0μmol/L,烯效唑浓度为1.00 mg/L时,东农425愈伤组织的诱导率最高,CuSO4浓度为15.0μmol/L,烯效唑浓度为1.00 mg/L时,东农425愈伤组织的分化率最高。
In order to establish a high-frequency regeneration system for mature embryos of cold japonica rice, maturing embryos were cultivated in cold-growing rice Kong-yu-131 and Dongnong-425 to study the effects of CuSO4 and uniconazole on the maturation of rice embryos . The results showed that the addition of 15.0μmol / L Cu2 + or 0.50 mg / L uniconazole to the culture medium could promote the induction and differentiation of mature embryos in cold region. CuSO4 And uniconazole, when the concentration of CuSO4 was 20.0μmol / L and the concentration of uniconazole was 1.00 mg / L, the induction rate and differentiation rate of Calluses reached the highest; the concentration of CuSO4 was 10.0μmol / L, When the concentration of uniconazole was 1.00 mg / L, the induction rate of Dongnong 425 was the highest, and the differentiation rate of Dongnong 425 was the highest when CuSO4 concentration was 15.0 μmol / L and uniconazole concentration was 1.00 mg / L highest.