论文部分内容阅读
目的:观察碱性成纤维细胞生长因子(bFGF)能否通过血迷路屏障,为临床合理用药提供实验依据。方法:豚鼠30只分为3组:125I-bFGF腹腔注入(125I-bFGF组)10只,125I腹腔注入(125I组)10只,生理盐水腹腔注入(生理盐水组)10只。2h后,取血、脑、双耳蜗、甲状腺、心脏、肝脏、肾脏称重,于r放射免疫分析仪中测定每个样品的每分钟记数率。双耳蜗标本切片做放射自显影检查。结果:125I组各脏器每分钟放射含量最高,耳蜗标本切片放射自显影可见颗粒显现;125I-bFGF组各脏器每分钟放射含量以血中最高,耳蜗及脑内含量最低,耳蜗标本切片放射自显影未见颗粒显现。结论:125I标记的bFGF腹腔注入后透过血迷路屏障有一定困难,因此,bFGF临床用药途径值得探讨。
OBJECTIVE: To investigate whether bFGF can pass the blood-labyrinth barrier and provide experimental basis for clinical rational use of drugs. Methods: Guinea pigs were randomly divided into three groups: 10 mice in 125I-bFGF group (125I-bFGF group), 10 mice in 125I group (125I group) and 10 mice in saline group (NS group). After 2 hours, the blood, brain, cochlear cochlear, thyroid, heart, liver and kidney were weighed and the per minute count rate of each sample was determined by r radioimmunoassay. Cochlear cochlear autotopic autopsy. Results: The radioactivity per minute of 125I-bFGF group was the highest in every organ of 125I-bFGF group. The content of radioactivity in each group of 125I-bFGF group was the highest in blood, the lowest in cochlear and brain, No self-developed particles appear. Conclusion: 125I-labeled bFGF is difficult to penetrate the blood-labyrinth barrier after intraperitoneal injection. Therefore, the clinical application of bFGF is worth exploring.