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目的:建立用高效液相色谱(HPLC)法测定腰腿痛丸中盐酸麻黄碱含量的方法。方法:以乙腈-0.1%磷酸(9:91)为流动相,VP-ODS C_(18)(250 mm×4.6 mm,5μm)为固定相,检测波长为207 nm。结果:盐酸麻黄碱在0.08～2.02μg的范围内线性关系良好(r=0.999 9),平均回收率为97.4%,RSD为1.9%。结论:该法快捷、简便、准确,能更好的控制其质量。
Objective: To establish a method for the determination of ephedrine hydrochloride in waist and leg pain pills by high performance liquid chromatography (HPLC). Methods: Acetonitrile-0.1% phosphoric acid (9:91) was used as the mobile phase. VP-ODS C 18 (250 mm×4.6 mm, 5 μm) was used as the stationary phase. The detection wavelength was 207 nm. RESULTS: Ephedrine hydrochloride had a good linearity in the range of 0.08-2.02 μg (r=0.999 9). The average recovery was 97.4% and the RSD was 1.9%. Conclusion: This method is quick, simple, accurate, and can better control its quality.