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通过原位合成法以金纳米种子作为母核诱导纳米金在其表面原位生长生成爆米花型金纳米粒子,标记甲胎蛋白抗体(anti-AFP)和辣根过氧化物酶(HRP)结合形成免疫复合物(antiAFP@popcorn-shaped GNP@HRP)作为检测抗体。氧化石墨烯-亚甲基蓝-金纳米粒子(GOM B-AuNPs)纳米复合材料滴涂到玻碳电极(GCE)表面,形成纳米复合物用于捕获甲胎蛋白抗体构建了免疫传感平台(anti-AFP/GO-MB-AuNPs/GCE)。实验采用夹心型免疫分析模式,功能化爆米花型金纳米材料与石墨烯纳米复合物呈现出良好的性能,建立了一种可行的电流型免疫分析法用于灵敏分析血清样品中甲胎蛋白AFP。在优化的实验条件下,免疫传感器DPV电流响应与AFP浓度的对数值呈正比,线性范围为0.001~20 ng/mL,检测限为0.31 pg/mL(S/N=3)。制备的免疫传感器有良好的精密度,选择性和稳定性,可初步应用于临床检验中AFP测定。
In situ synthesis of gold nanoparticles as the core to induce the growth of gold nanoparticles in situ on the surface of popcorn-type gold nanoparticles, labeling of anti-AFP and horseradish peroxidase (HRP) binding AntiAFP (popcorn-shaped GNP @ HRP) was used as the detection antibody. (GMA) nanocomposites were coated on the surface of glassy carbon electrode (GCE) to form nanocomposites for capture of α-fetoprotein antibody. The anti-AFP (anti-AFP) / GO-MB-AuNPs / GCE). Sandwich-type immunoassay was used in the experiment. Functionalized popcorn-type gold nanomaterials and graphene nanocomposites exhibited good performance. A viable current-mode immunoassay was developed for the sensitive analysis of AFP . Under optimized experimental conditions, the DPV current response of the immunosensor was proportional to the logarithm of the AFP concentration, with a linear range of 0.001 to 20 ng / mL and a detection limit of 0.31 pg / mL (S / N = 3). The prepared immunosensor has good precision, selectivity and stability and can be initially applied to AFP determination in clinical examination.