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目的 观察选择性环氧化酶2 (COX 2 )抑制剂氮2,环己氧4,硝基苯甲基磺胺(NS398)在诱导COX 2表达阴性的前列腺癌PC 3细胞凋亡中的作用。 方法 应用RT PCR和Westernblot的方法对PC 3细胞mRNA和蛋白水平的COX 2表达情况进行检测,四甲基偶氮唑蓝快速比色法观察不同浓度和时间NS398对PC 3细胞生存率的影响,流式细胞术检测100μmol/LNS398作用24hPC 3细胞的凋亡情况。 结果 mRNA和蛋白水平PC 3细胞COX 2均呈阴性表达;NS398可以抑制PC 3细胞的存活,并随浓度增加而增强,但无显著的时间依赖性;与对照组(10. 563±2. 582)%相比, 100μmol/LNS398诱导PC 3细胞早期凋亡比率(19. 307±3. 773)%显著增加,差异有统计学意义(P=0. 01)。 结论 选择性COX 2抑制剂NS398可以诱导COX 2表达阴性的PC 3细胞凋亡,提示COX 2非依赖性途径的存在。
Objective To observe the effects of selective cyclooxygenase 2 (COX 2) inhibitors such as nitrogen 2, cyclooxygenase 4 and nitrobenzylsulfonamide (NS398) on the apoptosis of prostate cancer PC 3 cells induced by COX 2. Methods The mRNA and protein expression of COX 2 in PC 3 cells were detected by RT PCR and Western blotting. The effect of NS398 at different concentrations and times on the survival rate of PC 3 cells was observed by MTT assay. Flow cytometry was used to detect the apoptosis of 24hPC 3 cells treated with 100μmol / L NS398. Results The mRNA and protein levels of COX 2 in PC 3 cells were negatively expressed. NS398 inhibited the survival of PC 3 cells and increased with increasing concentration, but no significant time-dependent manner. Compared with the control group (10. 563 ± 2. 582 )%, The rate of early apoptosis of PC 3 cells induced by 100μmol / L NS398 (19.37 ± 3.773%) was significantly increased, the difference was statistically significant (P = 0.01). Conclusion NS398, a selective COX 2 inhibitor, induces apoptosis of PC 3 cells that are negative for COX 2 expression, suggesting the existence of COX 2-independent pathways.