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目的检测SARS病变组织标本中的细胞凋亡情况和凋亡细胞类型,探讨细胞凋亡在SARS发病过程中的作用及可能机制。方法DNA片段原位末端检测和免疫组化碱性磷酸酶法单染和双染检测正常组织和SARS病变组织中的细胞凋亡以及白细胞分化相关抗原CD3、CD4、CD8、CD45RA、CD20、CD68的表达情况。结果与正常组织相比,SARS病人的脾脏、肺组织和淋巴结细胞凋亡明显增多,凋亡细胞包括肺泡上皮细胞、淋巴细胞和单核/巨噬细胞等;各病变组织内均有大量CD68+单核/巨噬细胞;病变肺组织基本没有CD45RA+/CD20+ B细胞和CD4+/CD8+T细胞浸润,淋巴结和脾脏的CD4+/CD8+T细胞、CD45RA+/CD20+B细胞也明显减少,现存的具有上述抗原特性的T细胞和B细胞较少凋亡。结论(1)细胞凋亡现象在SARS病变组织中普遍存在,提示细胞凋亡在SARS发病过程中起一定作用;(2)病变组织内浸润的炎细胞主要为单核细胞,说明单核细胞可能在SARS发病中起重要作用;(3)淋巴结和脾脏的细胞凋亡现象和CD4+/CD8+T细胞及CD45RA+/CD20+ B细胞减少提示SARS病毒可能对免疫细胞有一定的杀伤效应。
Objective To detect the apoptosis and the types of apoptotic cells in the tissue samples of SARS and to explore the possible role of apoptosis in the pathogenesis of SARS. Methods The DNA fragments were detected by end-to-end and immunohistochemical method of alkaline phosphatase and single staining and double staining for the detection of apoptosis and expression of leukocyte differentiation related antigen CD3, CD4, CD8, CD45RA, CD20 and CD68 in normal and SARS tissues Express the situation. Results Compared with normal tissues, the apoptosis of spleen, lung and lymph node in patients with SARS was significantly increased. The apoptotic cells included alveolar epithelial cells, lymphocytes and monocytes / macrophages. In each lesion, there were a large number of CD68 + There was no infiltration of CD45RA + / CD20 + B cells and CD4 + / CD8 + T cells in the diseased lung tissue. The CD4 + / CD8 + T cells and CD45RA + / CD20 + B cells in lymph nodes and spleens were also significantly reduced. Antigen characteristics of T cells and B cells less apoptosis. Conclusions (1) Apoptosis is common in SARS patients, suggesting that apoptosis plays a role in the pathogenesis of SARS. (2) The infiltrating inflammatory cells in the diseased tissues are mainly mononuclear cells, indicating that monocytes may be (3) Apoptosis of lymph nodes and spleens and the decrease of CD4 + / CD8 + T cells and CD45RA + / CD20 + B cells suggest that SARS virus may have certain killing effect on immune cells.