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目的:鼻咽癌(nasopharyngeal carcinoma,NPC)已成为我国头颈肿瘤患者的主要病死因素之一,局部治疗失败和远处转移是晚期NPC患者不良预后的主要原因.大量研究证明,ADHlB是多种癌症的风险基因,本研究探讨ADHlB在鼻咽癌细胞中的表达差异以及对鼻咽癌细胞增殖和迁移能力的影响.方法: 利用生物信息学方法分析 ADHlB在头颈鳞状细胞癌( head and neck squamous cell carcinoma,HNSC)与正常组织中的相对表达量,应用实时荧光定量PCR(qRT-PCR)检测正常永生化鼻咽上皮细胞NP69及鼻咽癌细胞CNE-1、CNE-2、5-8F、6-10B中ADHlB的mRNA表达水平,对比两种研究结果的一致性.在此基础上建立稳转ADHlB高表达鼻咽癌细胞系,以pCDH作为空载体对照.利用生长曲线实验检验鼻咽癌细胞的增殖能力,利用划痕实验检验鼻咽癌细胞的迁移能力.结果:生物信息学分析结果显示ADHlB在头颈鳞状细胞癌中表达量低于正常组织. qRT-PCR检测发现,ADHlB在鼻咽癌细胞CNE-1、CNE-2、5-8F、6-10B中均比NP69细胞表达低,过表达ADHlB组鼻咽癌细胞的生长曲线低于pCDH组(P<0. 05),划痕愈合速度也低于正常表达组pCDH组( P<0. 05).结论:鼻咽癌细胞中ADHlB基因表达异常,明显低于正常鼻咽上皮细胞,与生物信息学分析结果一致. ADHlB基因在鼻咽癌中高表达影响鼻咽癌细胞的增殖和迁移,这与NPC的发生发展密切相关,可能是参与调控NPC发生发展的一个重要靶标.“,”Nasopharyngeal carcinoma (NPC) has become the main cause of death in head and neck cancer in China. Failed local treatment and distant metastasis are the main causes of poor prognosis in patients with advanced NPC. A large number of studies have shown that ADHlB is a risk gene for a variety of cancers. This study investigated the differential expression of ADHlB in NPC cells and its effect on the proliferation and migration of NPC cells. Methods: Bioinformatics analysis was performed to compare the relative expression of ADHlB in head and neck squamous cell carcinoma (HNSC) and normal tissue. Quantitative real-time PCR (qRT-PCR) was used to detect the mRNA expression level of ADHlB in NP69, a normal immortalized nasopharyngeal epithelial cell, and in CNE-1, CNE-2, 5-8F and 6-10B, NPC cells. On this basis, a stable NPC cell line which highly expresses ADHlB and a pCHD empty vector control was established. The growth curve experiment was used to test the proliferative ability of NPC cells, and the scratch wound healing assay was used to test the migration ability of NPC cells. Results: Bioinformatics analysis showed that ADHlB expression in HNSC was lower than that in normal tissue. ADHlB expression was lower in CNE-1, CNE-2, 5-8F, and 6-10B than in NP69 cells. The proliferation of NPC cells in the over-expressed ADHlB group was lower than that in the pCDH group ( P<0. 05), and the wound healing rate in the former was lower than that in the latter (P<0. 05). Conclusion: The expression of ADHlB in NPC cells was significantly lower than that in normal nasopharyngeal epithelial cells, which was consistent with results of bioinformatics anal-ysis. The high expression of ADHlB in NPC affects the proliferation and migration of NPC cells, which is closely related to and may be an important target for the occurrence and development of NPC.