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目的探讨仙鹤草抗脑缺血再灌注损伤的作用机制,筛选有效部位。方法采用改良MCAO法制备脑缺血再灌注损伤模型,将SD大鼠随机分为假手术组、模型组、仙鹤草乙酸乙酯提取物组(4 g/kg)、仙鹤草乙醇提取物组(4 g/kg)、仙鹤草水提取物组(4 g/kg)、尼莫地平组(0.5 mg/kg)。分别于再灌注0、6 h分组灌胃给药,观察各组对脑缺血再灌注引起的神经功能缺损症状、脑梗死体积及脑组织病理学(透射电镜)的调控作用,以及对脑组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)的调控作用,探讨其抗脑缺血再灌注损伤的作用机制,筛选不同提取物中的有效部位。结果仙鹤草水提取物能明显减轻大鼠的神经功能缺损症状(P<0.05);通过TTC染色及电镜观察,仙鹤草水提取物能明显缩小脑梗死体积(P<0.01)及改善大鼠大脑皮质神经元病变,提高大鼠脑缺血再灌后SOD,显著降低MDA含量和NO水平(P<0.05)。结论仙鹤草水提取物可能成为抗脑缺血再灌注损伤有效部位,其作用机制可能为抑制脂质过氧化反应、增加缺血区脑组织能量物质含量,最终达到防止脑组织细胞损伤的作用。
Objective To investigate the mechanism of agrimony against cerebral ischemia-reperfusion injury and screen effective sites. Methods The model of cerebral ischemia-reperfusion injury was established by modified MCAO. SD rats were randomly divided into sham-operation group, model group, ethyl acetate extracts of Agrimony (4 g / kg), ethanol extract of Agrimony group 4 g / kg), Agrimony water extract (4 g / kg) and nimodipine (0.5 mg / kg). The rats were intragastrically administrated at 0 and 6 h after reperfusion, respectively. The effects of cerebral ischemia-reperfusion-induced neurological impairment, cerebral infarction volume and brain histopathology (TEM) were observed. (SOD), malondialdehyde (MDA) and nitric oxide (NO) in rats and explore their mechanism of action against cerebral ischemia-reperfusion injury and to screen the effective fractions of different extracts. Results Water extract of Agrimony can significantly reduce the symptoms of neurological deficits in rats (P <0.05). By TTC staining and electron microscopy, Agrimony water extracts can significantly reduce the volume of cerebral infarction (P <0.01) and improve the rat brain Cortical neuron lesion, increase of SOD after cerebral ischemia-reperfusion in rats, significantly reduce the content of MDA and NO (P <0.05). Conclusion Agrimony extract may be an effective anti-cerebral ischemia-reperfusion injury mechanism. Its mechanism may be to inhibit lipid peroxidation and increase the energy and substance content of ischemic brain tissue, and finally to prevent the damage of brain cells.