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生物质炭作为一种新型土壤改良剂,施入土壤不仅能提高肥力,改善土壤结构,还能够影响土壤氮素的转化.本文利用培养试验研究施用生物质炭对采煤塌陷区土壤性质及氨氧化菌丰度和群落结构的影响.结果表明:生物质炭显著提高土壤铵氮(NH_4~+-N)、全氮、有效磷和速效钾含量.生物质炭施用量对氨氧化古菌(AOA)丰度没有显著影响,但是增加施用量显著提高了氨氧化细菌(AOB)丰度.对T-RFLP数据进行分析发现,生物质炭提高了AOA和AOB多样性,并在一定程度上改变了AOA和AOB群落结构.施用生物质炭提高了采煤塌陷区土壤养分含量,并在一定程度上提高了氨氧化菌的丰度和多样性,表明生物质炭对塌陷区复垦土壤具有培肥改良的潜能.
As a new soil conditioner, biomass charcoal can not only improve fertility, improve soil structure, but also affect the transformation of soil nitrogen.In this paper, culture experiments were conducted to study the effect of biomass carbon on the soil properties and ammonia The results showed that the biomass carbon significantly increased the content of NH_4 ~ + -N, total nitrogen, available phosphorus and available potassium in soil, AOA abundance, there was no significant effect, but increasing the application rates significantly increased AOB abundance.Analysis of T-RFLP data found that biomass carbon increased AOA and AOB diversity to some extent AOA and AOB community structure.The application of biochar increased soil nutrient content in subsidence area and increased the abundance and diversity of ammonia oxidizing bacteria to a certain extent, Fertilizer to improve the potential.