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目的建立一种酶联免疫法来检测病人体内的特异性ASC,据此判断其体内是否有活动性梅毒螺旋体感染。方法采用梅毒重组融合抗原ELISA试剂盒分别检测正常人群、潜伏期梅毒、一期梅毒、二期梅毒患者的特异性ASC。结果各病例组均检出特异性ASC,一期梅毒组的OD均值明显高于二期梅毒组和潜伏梅毒组,差异具有显著性(P<0 01)。GST-epi-TpN17抗原检测灵敏度优于GST-TpN17融合抗原。结论本研究建立的梅毒螺旋体特异性ASC检测方法可用于活动性梅毒感染的诊断以及作为疗效观察的指标,为临床检测梅毒感染提供了新思路。
Objective To establish an enzyme-linked immunosorbent assay (ELISA) for the detection of specific ASC in patients and to determine whether there is active Treponema pallidum infection in the body. Methods The syphilis recombinant fusion antigen ELISA kit was used to detect the specific ASC of normal population, latent syphilis, primary syphilis and secondary syphilis respectively. Results The specific ASC was detected in all the cases. The mean OD of the first stage syphilis group was significantly higher than that of the second stage syphilis group and the latent syphilis group (P <0.01). GST-epi-TpN17 antigen detection sensitivity than GST-TpN17 fusion antigen. Conclusion The detection method of Treponema pallidum specific ASC established in this study can be used for the diagnosis of active syphilis infection and as an indicator of efficacy, which provides a new idea for the clinical detection of syphilis infection.