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目的:研究抑癌基因 wtp53在不同发育时期 Wistar 大鼠小肠上皮细胞的表达、分布特征以及与细胞增殖分化的关系,探讨 wtp53基因在小肠创伤修复时可能发挥的生物学作用。方法:利用 wtp53单克隆抗体,以超敏的 SP 免疫组织化学方法,观察 wtp53基因在胚胎、新生期各时间段 Wistar 大鼠小肠上皮细胞的阳性表达与分布特征。同时以细胞增殖活性的标志 PCNA 表达为参照,对不同时期细胞的增殖分化状况作对比性研究。结果:在胚胎第17d(E17d)、E19d、新生期第1(P1)d,小肠上皮细胞 wtp53基因的阳性表达范围较广,染色强度较深。P2d和 wtp53基因表达不仅范围扩大且染色强度明显增强;到幼鼠期第28d,wtp53基因在小肠上皮细胞的表达为阴性;从胚胎至 P2d,PCNA 的表达与 wtp53类似,而幼鼠期第28d,PCNA 阳性细胞仅局限于小肠绒毛隐窝。结论:在小肠发育的早期,上皮细胞的快速增殖分化期伴随着 wtp53基因的高度表达,可能对遗传物质 DNA的完整性起到监视和保护作用。
OBJECTIVE: To study the expression and distribution of wtp53 gene in small intestine epithelial cells of Wistar rats at different developmental stages and its relationship with cell proliferation and differentiation, and to explore the biological role that wtp53 may play in the repair of small intestinal trauma. Methods: The positive expression and distribution of wtp53 gene in small intestine epithelial cells of Wistar rats at embryonic and neonatal stages were observed by using hypersensitive SP immunohistochemistry with wtp53 monoclonal antibody. At the same time, the cell proliferation activity of PCNA expression as a reference, the proliferation and differentiation of cells at different stages for comparative study. Results: On day 17 (E17d), day E19 (d), on day 1 (P1) d, the wtp53 gene in small intestine epithelial cells had a wide range of positive expression and dark staining. The expression of wtp53 and wtp53 were not only broadened and the staining intensity was significantly increased. The expression of wtp53 gene in intestinal epithelial cells was negative at 28th day of gestation. The expression of PCNA was similar to wtp53 from embryo to P2d, , PCNA-positive cells are confined to the intestinal villous crypts. CONCLUSION: In the early stage of small intestine, the rapid proliferation and differentiation of epithelial cells accompanied by the high expression of wtp53 gene may play a role in monitoring and protecting the integrity of DNA of genetic material.