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[目的]建立贞芪扶正颗粒中特女贞苷含量测定方法。[方法]采用Thermo Syncronis C18(4.6×250mm,5μm)、Agilent ZORBAX SB-AQ(4.6×250mm,5μm)、SPOLAR C18(4.6×250mm,5μm);十八烷基硅烷键合硅胶为填充剂-C18(4.6×250mm,5μm);柱温:30℃;检测波长:224nm;流动相:甲醇-水(40:60);流速:1.0m L/min-1。[结果]精密度RSD=0.4%,重复性RSD=1.0%,稳定性RSD=0.9%,耐用性理论板数均>3000,加样回收率RSD=0.78%。限定贞芪扶正颗粒中特女贞苷含量不得低于0.21mg/g。[结论]高效液相色谱法测定贞芪扶正颗粒特女贞苷含量,结果准确、可靠,可用于贞芪扶正颗粒质量控制。
[Objective] To establish a method for determination of the telmisartan in Zhenqi Fuzheng granule. [Method] Thermo Syncronis C18 (4.6 × 250mm, 5μm), Agilent ZORBAX SB-AQ (4.6 × 250mm, 5μm) and SPOLAR C18 (4.6 × 250mm, C18 (4.6 × 250 mm, 5 μm); column temperature: 30 ° C .; detection wavelength: 224 nm; mobile phase: methanol-water (40:60); flow rate: 1.0 m L / min-1. [Result] The precision of RSD was 0.4%, the repeatability of RSD was 1.0%, the stability of RSD was 0.9%, the number of durability theory plates was more than 3000, and the recovery rate was RSD 0.78%. Limits Zhenzheng Fuzheng particles in the special virginia glycosides content of not less than 0.21mg / g. [Conclusion] The HPLC method for the determination of virginia glycosides in Zhenqi Fuzheng Granules was accurate and reliable and could be used for the quality control of Zhenqi Fuzheng Granules.